{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Zheng Min"],"instrument_platform":["HiSeq X Ten"],"study_type":["RNA-seq of coding RNA"],"organism":["Epinephelus fuscoguttatus x Epinephelus lanceolatus"],"species":["Epinephelus fuscoguttatus x Epinephelus lanceolatus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15515"],"description":["Edwardsiella ictaluri, a significant pathogen in aquaculture, can lead to severe intestinal damage and metabolic disorders in fish. Verification via Transcriptome Sequencing of the intestinal damage caused by Edwardsiella ictaluri in Hybrid Groupers."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - cDNA libraries were constructed as per the instruction manual of NEBNext Ultra RNA Library Prep Kit.","Nucleic Acid Extraction - Total RNA was extracted from the midgut of  hybrid groupers by using the TRlzol Reagent and following the manufacturer’s instructions (Life Technologies, California, USA).","Sample Collection - Before the start of the experiment, 600 healthy hybrid groupers with an average body weight of 42.3 ± 2.8 g were acclimatized in a recirculating aquaculture system (water temperature: 28–30°C; pH: 7.8 ± 0.2) and fasted for 24 h. The fish were then randomly allocated to two treatment groups: Control group ( con)which received no treatment; E. ictaluri-infected group (ADH) which was administered E. ictaluri via oral gavage.","Sequencing - These built cDNA libraries were sequenced on an Illumina Hi-Seq X Ten system by the OE Biotech Co., Ltd. (Shanghai, China)."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Zheng Min"],"additional_accession":[]},"is_claimable":false,"name":"Modulation of midgut immune-metabolic pathway in hybrid grouper infected with Edwardsiella ictaluri","description":"Edwardsiella ictaluri, a significant pathogen in aquaculture, can lead to severe intestinal damage and metabolic disorders in fish. Verification via Transcriptome Sequencing of the intestinal damage caused by Edwardsiella ictaluri in Hybrid Groupers.","dates":{"release":"2025-09-15T00:00:00Z","modification":"2025-09-15T01:02:30.725Z","creation":"2025-08-31T20:02:40.108Z"},"accession":"E-MTAB-15515","cross_references":{"ENA":["ERP179411"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}