{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Rui Guo"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15517"],"description":["Using RNA sequencing (RNA-seq), assessed the impact of OGT deficiency in germ cells on mRNA metabolism in mouse testes."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - The libraries were sequenced on an Illumina Novaseq 6000 platform and 150 bp paired-end reads were generated.","Library Construction - Then the libraries were constructed using VAHTS Universal V6 RNA-seq Library Prep Kit according to the manufacturer’s instructions. The transcriptome sequencing and analysis were conducted by OE Biotech Co., Ltd. (Shanghai, China).","Nucleic Acid Extraction - Total RNA was extracted using the TRIzol reagent (Invitrogen, CA, USA) according to the manufacturer’s protocol. RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).","Sample Collection - Fresh mouse testes were dissected, with adherent adipose tissue carefully removed, washed twice in PBS, flash-frozen in liquid nitrogen, and stored for subsequent RNA extraction."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - The clean reads were mapped to the reference genome using HISAT2 . FPKM  of each gene was calculated and the read counts of each gene were obtained by HTSeq-count ."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000","Illumina Nova 6000 PE150"],"study_type":["RNA-seq of total RNA"],"species":["Mus musculus"],"pubmed_authors":["Rui Guo"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of P10 (postnatal day 10) testes in Wild-type and Ogt cKO mice","description":"Using RNA sequencing (RNA-seq), assessed the impact of OGT deficiency in germ cells on mRNA metabolism in mouse testes.","dates":{"release":"2026-08-01T00:00:00Z","modification":"2026-08-01T01:01:00.696Z","creation":"2025-08-29T05:28:19.515Z"},"accession":"E-MTAB-15517","cross_references":{"ENA":["ERP179377"],"EFO":["EFO_0002944","EFO_0004170","EFO_0009653","EFO_0005518","EFO_0003816","EFO_0004184"]}}