{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Colette Tournaire Roux"],"study_type":["transcription profiling by array"],"organism":["Arabidopsis thaliana"],"species":["Arabidopsis thaliana"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15518"],"description":["SnRK2 have  been identified as componant in plant hormonal and environmental stress signaling.. Although responsive to both ABA and osmotic stress, SnRK2s from class III were proposed to act as the main regulators of ABA signaling whereas class I would be exclusively responsive to osmotic stress.  Strikingly, knock-out mutations of all class III SnRK2s induce dramatic phenotypes showing little or no responses to high ABA concentrations during seed germination, root growth, and stomatal movement. On the other hand, class I SnRK2s (SnRK2.1, 2.4, 2.5, 2.9, 2.10) have been generally considered as ABA-unresponsive and rather responding to salinity and osmotic stress. Yet, heterologous expression studies in yeast aiming to test the functionality of a variety of core ABA signaling components, indicated that SnRK2.4 may be involved in ABA-dependent signaling. In support for this, a recent study revealed the role of SnRK2.4 in ABA-dependent down regulations of root aquaporins activity and consequent reduction of water transport to aerial parts . The present study addresses the responses of SnRK2.4 to ABA with regard to root development and membrane cellular functions. For this, we developed an integrated transcriptomic, proteomic and phosphoproteomic study of two snrk2.4 knock-out mutants, treated by 1µM ABA for 3H."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - SV Total RNA Isolation System followed by RQ1 DNASe traetment","Growth Protocol - seeds were grown on plate with MS/2 medium for 11  x days  and transferred to hydroponic solution in for 11 days.","Scaning - arrays were scanned in a GeneAtlas® Imaging Station. The Imaging Station images the fluorescent labeled array and converts the intensity information into .cel files","Hybridization - GeneAtlasTM Hybridization, Wash, and Stain Kit for WT Array Strips , in The GeneAtlas® Fluidics Station","Labeling - samples were labeled with biotin using GeneChip® WT Terminal Labeling Kit","Sample Collection - Roots from 22 days plants cultured in hydroponic conditions were collected.","Sample Treatment - 1 µM ABA (in ethanol) was added to roots grown in hydroponics for 11 days (22 days old ) for 3 hours before roots harvesting."],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Colette Tournaire Roux"],"data_protocol":["Data Transformation - The summerization method gene-level RMA of Affymetrix ®GeneChip® Operating Software was used"],"additional_accession":[]},"is_claimable":false,"name":"microarrays expression of Arabidopsis thaliana roots treated by ABA (1µM for 3H) in Col0 control and snrk2.4 knock-out  against non treated roots","description":"SnRK2 have  been identified as componant in plant hormonal and environmental stress signaling.. Although responsive to both ABA and osmotic stress, SnRK2s from class III were proposed to act as the main regulators of ABA signaling whereas class I would be exclusively responsive to osmotic stress.  Strikingly, knock-out mutations of all class III SnRK2s induce dramatic phenotypes showing little or no responses to high ABA concentrations during seed germination, root growth, and stomatal movement. On the other hand, class I SnRK2s (SnRK2.1, 2.4, 2.5, 2.9, 2.10) have been generally considered as ABA-unresponsive and rather responding to salinity and osmotic stress. Yet, heterologous expression studies in yeast aiming to test the functionality of a variety of core ABA signaling components, indicated that SnRK2.4 may be involved in ABA-dependent signaling. In support for this, a recent study revealed the role of SnRK2.4 in ABA-dependent down regulations of root aquaporins activity and consequent reduction of water transport to aerial parts . The present study addresses the responses of SnRK2.4 to ABA with regard to root development and membrane cellular functions. For this, we developed an integrated transcriptomic, proteomic and phosphoproteomic study of two snrk2.4 knock-out mutants, treated by 1µM ABA for 3H.","dates":{"release":"2025-09-15T00:00:00Z","modification":"2025-09-15T01:02:33.06Z","creation":"2025-08-29T11:22:43.118Z"},"accession":"E-MTAB-15518","cross_references":{"EFO":["EFO_0002768","EFO_0002944","EFO_0003814","EFO_0003813","EFO_0003789","EFO_0005518","EFO_0003816","EFO_0003815","EFO_0003969"]}}