{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Stefan Wiemann"],"organism":["Homo sapiens"],"software":["Rnbeads (v.2.21.3)"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15519"],"description":["MCF7 (parental) cells were cultivated in estrogen deprived media for 12 months (long-term estrogen deprivation - LTED), until they had regained proliferative capacity (proxy for resistance to aromatase inhibition). Methylomes of parental and LTED MCF7 cells were analyzed to identify differentially methylated regions."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Scaning - Quality control, filtering and normalization were performed using Rnbeads (v.2.21.3)","Sample Treatment - MCF7 long term estrogen deprived (LTED) cells were cultivated in media lacking estrogen and phenol red.","Hybridization - Methylation profiling was performed by the DKFZ Microarray Core Facility using the Illumina MethylationEPIC BeadChip platform (Illumina, San Diego, CA, USA).","Sample Collection - Parental MCF7 cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% FCS, 50 units/mL penicillin, 50 µg/mL streptomycin sulfate (Invitrogen AG, Carlsbad, CA, USA) and incubated at 37°C with 5% CO2 in a humidified incubator. Media was supplemented with 10−8 M 17-ß-estradiol (E2, Sigma-Aldrich, Saint-Louis, MI, USA) for cultivation of MCF7 parental cell parental line. Long-term estrogen-deprived (LTED) cells were cultured for one year in estrogen-free DMEM (w/o phenolred) supplemented with 10% charcoal-stripped FCS until the cells had regained proliferative capacities similar to parental MCF7 cells.","Nucleic Acid Extraction - Total DNA was extracted from MCF7 parental and LTED cells using the DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany), following the manufacturer’s protocol.","Labeling - Methylation profiling was performed by the DKFZ Microarray Core Facility using the Illumina MethylationEPIC BeadChip platform (Illumina, San Diego, CA, USA)."],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","MAGE-TAB Files","Array Designs"],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina MethylationEPIC BeadChip platform (Illumina, San Diego, CA, USA)"],"study_type":["methylation profiling by array"],"species":["Homo sapiens"],"pubmed_authors":["Stefan Wiemann"],"additional_accession":[]},"is_claimable":false,"name":"Methylome profiling of MCF7 parental and long-term estrogen deprived cell lines","description":"MCF7 (parental) cells were cultivated in estrogen deprived media for 12 months (long-term estrogen deprivation - LTED), until they had regained proliferative capacity (proxy for resistance to aromatase inhibition). Methylomes of parental and LTED MCF7 cells were analyzed to identify differentially methylated regions.","dates":{"release":"2025-10-27T00:00:00Z","modification":"2025-10-27T02:02:11.468Z","creation":"2025-08-29T11:20:33.82Z"},"accession":"E-MTAB-15519","cross_references":{"EFO":["EFO_0002944","EFO_0003814","EFO_0003813","EFO_0002759","EFO_0005518","EFO_0003815","EFO_0003969"]}}