biostudies-arrayexpressStefan WiemannHomo sapiensRnbeads (v.2.21.3)https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15519MCF7 (parental) cells were cultivated in estrogen deprived media for 12 months (long-term estrogen deprivation - LTED), until they had regained proliferative capacity (proxy for resistance to aromatase inhibition). Methylomes of parental and LTED MCF7 cells were analyzed to identify differentially methylated regions.biostudies-arrayexpressScaning - Quality control, filtering and normalization were performed using Rnbeads (v.2.21.3)Sample Treatment - MCF7 long term estrogen deprived (LTED) cells were cultivated in media lacking estrogen and phenol red.Hybridization - Methylation profiling was performed by the DKFZ Microarray Core Facility using the Illumina MethylationEPIC BeadChip platform (Illumina, San Diego, CA, USA).Sample Collection - Parental MCF7 cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% FCS, 50 units/mL penicillin, 50 µg/mL streptomycin sulfate (Invitrogen AG, Carlsbad, CA, USA) and incubated at 37°C with 5% CO2 in a humidified incubator. Media was supplemented with 10−8 M 17-ß-estradiol (E2, Sigma-Aldrich, Saint-Louis, MI, USA) for cultivation of MCF7 parental cell parental line. Long-term estrogen-deprived (LTED) cells were cultured for one year in estrogen-free DMEM (w/o phenolred) supplemented with 10% charcoal-stripped FCS until the cells had regained proliferative capacities similar to parental MCF7 cells.Nucleic Acid Extraction - Total DNA was extracted from MCF7 parental and LTED cells using the DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany), following the manufacturer’s protocol.Labeling - Methylation profiling was performed by the DKFZ Microarray Core Facility using the Illumina MethylationEPIC BeadChip platform (Illumina, San Diego, CA, USA).MIAME ScoreRaw DataOrganizationAssays and DataMAGE-TAB FilesArray DesignsUnknownTranscriptomicsGenomicsProteomicsIllumina MethylationEPIC BeadChip platform (Illumina, San Diego, CA, USA)methylation profiling by arrayHomo sapiensStefan WiemannfalseMethylome profiling of MCF7 parental and long-term estrogen deprived cell linesMCF7 (parental) cells were cultivated in estrogen deprived media for 12 months (long-term estrogen deprivation - LTED), until they had regained proliferative capacity (proxy for resistance to aromatase inhibition). Methylomes of parental and LTED MCF7 cells were analyzed to identify differentially methylated regions.2025-10-27T00:00:00Z2025-10-27T02:02:11.468Z2025-08-29T11:20:33.82ZE-MTAB-15519EFO_0002944EFO_0003814EFO_0003813EFO_0002759EFO_0005518EFO_0003815EFO_0003969