{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Denise Ragusa"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15526"],"description":["K562-t(7;12) cells (K562 background with CRISPR/Cas9 engineered t(7;12) translocation) were treated with SNX-2112 (210 nM), OSI930 (9.19 µM), or DMSO (control) (0.1%) for 24 hours."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - Library construction was performed by Novogene UK (Cambridge, UK). A minimum of 400 ng (20 ng/μl) of high-quality total RNA was supplied for sequencing. Novogene UK constructed cDNA libraries by purification of messenger RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, PCR amplification, and purification.","Sample Collection - K562-t(7;12) cells suspensions treated with each compounds were centrifuged at 400 x g for 5 minutes to pellet for subsequent extraction.","Sequencing - Sequencing was performed by Novogene UK (Cambridge, UK) on NovaSeq Plus PE150 platform, at 50M paired-end reads per sample.","Nucleic Acid Extraction - Total RNA was extracted using Monarch Total RNA Miniprep Kit (NEB)."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Cristina Pina","Denise Ragusa"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of engineered K562 cell line with t(7;12)(q36;p13) treated with SNX-2112 and OSI930","description":"K562-t(7;12) cells (K562 background with CRISPR/Cas9 engineered t(7;12) translocation) were treated with SNX-2112 (210 nM), OSI930 (9.19 µM), or DMSO (control) (0.1%) for 24 hours.","dates":{"release":"2025-09-12T00:00:00Z","modification":"2025-09-12T16:11:55.486Z","creation":"2025-09-02T19:53:32.837Z"},"accession":"E-MTAB-15526","cross_references":{"ENA":["ERP179523"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}