biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsDenise RagusaIllumina NovaSeq 6000RNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15526K562-t(7;12) cells (K562 background with CRISPR/Cas9 engineered t(7;12) translocation) were treated with SNX-2112 (210 nM), OSI930 (9.19 µM), or DMSO (control) (0.1%) for 24 hours.biostudies-arrayexpressLibrary Construction - Library construction was performed by Novogene UK (Cambridge, UK). A minimum of 400 ng (20 ng/μl) of high-quality total RNA was supplied for sequencing. Novogene UK constructed cDNA libraries by purification of messenger RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, PCR amplification, and purification.Sample Collection - K562-t(7;12) cells suspensions treated with each compounds were centrifuged at 400 x g for 5 minutes to pellet for subsequent extraction.Sequencing - Sequencing was performed by Novogene UK (Cambridge, UK) on NovaSeq Plus PE150 platform, at 50M paired-end reads per sample.Nucleic Acid Extraction - Total RNA was extracted using Monarch Total RNA Miniprep Kit (NEB).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesCristina PinaDenise RagusafalseRNA-seq of engineered K562 cell line with t(7;12)(q36;p13) treated with SNX-2112 and OSI930K562-t(7;12) cells (K562 background with CRISPR/Cas9 engineered t(7;12) translocation) were treated with SNX-2112 (210 nM), OSI930 (9.19 µM), or DMSO (control) (0.1%) for 24 hours.2025-09-12T00:00:00Z2025-09-12T16:11:55.486Z2025-09-02T19:53:32.837ZE-MTAB-15526ERP179523EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184