{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Denise Ragusa"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15530"],"description":["K562 cells with a CRISPR-engineered t(7;12)(q36;p13) translocation (K562-t(7;12)) and control line transiently transfected with Cas9 only (K562-ctrl) were FACS sorted on positivity to surface markers Kit and CD24."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - FACS sorted cells were centrifuged at 400 x g for 5 minutes to pellet for subsequent extraction.","Library Construction - Library construction was performed by Novogene UK (Cambridge, UK). A minimum of 400 ng (20 ng/μl) of high-quality total RNA was supplied for sequencing. Novogene UK constructed cDNA libraries by purification of messenger RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, PCR amplification, and purification.","Sequencing - Sequencing was performed by Novogene UK (Cambridge, UK) on NovaSeq Plus PE150 platform, at 50M paired-end reads per sample.","Nucleic Acid Extraction - Total RNA was extracted using Monarch Total RNA Miniprep Kit (NEB)."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Cristina Pina","Denise Ragusa"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of Kit+CD24+ fractions in K562-t(7;12) compared to K562-control","description":"K562 cells with a CRISPR-engineered t(7;12)(q36;p13) translocation (K562-t(7;12)) and control line transiently transfected with Cas9 only (K562-ctrl) were FACS sorted on positivity to surface markers Kit and CD24.","dates":{"release":"2025-09-12T00:00:00Z","modification":"2025-09-12T16:11:21.61Z","creation":"2025-09-02T20:42:46.98Z"},"accession":"E-MTAB-15530","cross_references":{"ENA":["ERP179528"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}