biostudies-arrayexpressPaola VagnarelliHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15533Mitotic exit is an important part of the cell cycle that requires coordination of many chromatin and cytoskeleton remodelling events to successfully complete cell division and maintain cell identity. Protein de-phosphorylation is a key step in directing mitotic exit and protein phosphatase 1 (PP1) is essential to this process. However, the specific contribution of its numerous targeting subunits is still unknown. Here we have investigated the function of three chromatin-associated PP1 targeting subunits in exiting mitosis: Repo-Man, Ki-67 and PNUTS. We have generated endogenously tagged, auxin-degradable alleles for each subunit and used a multi-omic approach to address their specific contribution to transcription resumption, chromatin accessibility and protein phosphorylation at the transition from mitosis to G1. This approach has identified their distinct role in mitotic exit, provided unique datasets for the cell cycle community, and highlighted specific and novel functions for Ki-67 and Repo-Man in genome stability and organisation.biostudies-arrayexpressSample Collection - Cells were treated for 18 h with 2 mM thymidine before collection.Nucleic Acid Extraction - ChIP was performed and DNA was purified using AMPure XP beads (according to the manufacturer's protocol).Sequencing - ChIP sequencing was conducted from Novogene using the sequencing platform Illumina NovaSeq X Plus Series.Library Construction - Library preparation was conducted from Novogene using NEBNext® Ultra™ IIDNA Library Prep Kit.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Sequences were aligned to human genome Hg38 with bowtie2 with default parameters. High quality primary alignments were selected with samtools view with the parameters -F 260 and -q 20, and duplicates marked with samtools markdup. Bigwig profiles were generated using Deeptools bamCoverage and bigwigCompare at 500bp resolutionUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XChIP-seqHomo sapiensPaola VagnarelliKonstantinos StamatioufalseDifferential contribution of the chromatin-associated PP1 binding proteins PNUTS, Repo-Man and Ki-67 to mitotic exit and G1 re-establishment.Mitotic exit is an important part of the cell cycle that requires coordination of many chromatin and cytoskeleton remodelling events to successfully complete cell division and maintain cell identity. Protein de-phosphorylation is a key step in directing mitotic exit and protein phosphatase 1 (PP1) is essential to this process. However, the specific contribution of its numerous targeting subunits is still unknown. Here we have investigated the function of three chromatin-associated PP1 targeting subunits in exiting mitosis: Repo-Man, Ki-67 and PNUTS. We have generated endogenously tagged, auxin-degradable alleles for each subunit and used a multi-omic approach to address their specific contribution to transcription resumption, chromatin accessibility and protein phosphorylation at the transition from mitosis to G1. This approach has identified their distinct role in mitotic exit, provided unique datasets for the cell cycle community, and highlighted specific and novel functions for Ki-67 and Repo-Man in genome stability and organisation.2026-01-31T00:00:00Z2026-01-31T02:02:04.111Z2025-09-02T20:50:27.275ZE-MTAB-15533ERP179532EFO_0002944EFO_0004170EFO_0002692EFO_0005518EFO_0003816EFO_0004184