{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Betty Ying-Wen Chung"],"instrument_platform":["NextSeq 2000"],"study_type":["RNA-seq of coding RNA"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15534"],"description":["Parallel RNA- and Ribo-Seq of immortalised murine bone marrow derived macrophages following Salmonella infection. Cells were infected with WT or ΔprgJ Salmonella Typhimurium or mock infected at 60 min post infection."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Cells were lysed in buffer containing cycloheximide and chloramphenicol, and lysates were split for RNA-Seq and Ribo-Seq.","Sample Collection - iBMDM were infected with WT or ΔprgJ Salmonella Typhimurium or mock infected for 60 min.","Sequencing - Sequencing was performed using NextSeq-2000","Library Construction - For Ribo-Seq, lysates were treated with RNase I and fragments protected from digestion by the ribosome were purified. For RNA-Seq, total cellular RNA was fragmented by alkaline hydrolysis. Both produce short RNA fragments, from which libraries were generated."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Betty Ying-Wen Chung"],"additional_accession":[]},"is_claimable":false,"name":"Ribosome profiling and RNA-seq of Salmonella infected iBMDM","description":"Parallel RNA- and Ribo-Seq of immortalised murine bone marrow derived macrophages following Salmonella infection. Cells were infected with WT or ΔprgJ Salmonella Typhimurium or mock infected at 60 min post infection.","dates":{"release":"2025-08-28T00:00:00Z","modification":"2025-08-28T12:25:49.692Z","creation":"2025-08-28T09:49:06.748Z"},"accession":"E-MTAB-15534","cross_references":{"ENA":["ERP179251"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}