biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsBetty Ying-Wen ChungNextSeq 2000RNA-seq of coding RNAMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15534Parallel RNA- and Ribo-Seq of immortalised murine bone marrow derived macrophages following Salmonella infection. Cells were infected with WT or ΔprgJ Salmonella Typhimurium or mock infected at 60 min post infection.biostudies-arrayexpressNucleic Acid Extraction - Cells were lysed in buffer containing cycloheximide and chloramphenicol, and lysates were split for RNA-Seq and Ribo-Seq.Sample Collection - iBMDM were infected with WT or ΔprgJ Salmonella Typhimurium or mock infected for 60 min.Sequencing - Sequencing was performed using NextSeq-2000Library Construction - For Ribo-Seq, lysates were treated with RNase I and fragments protected from digestion by the ribosome were purified. For RNA-Seq, total cellular RNA was fragmented by alkaline hydrolysis. Both produce short RNA fragments, from which libraries were generated.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesBetty Ying-Wen ChungfalseRibosome profiling and RNA-seq of Salmonella infected iBMDMParallel RNA- and Ribo-Seq of immortalised murine bone marrow derived macrophages following Salmonella infection. Cells were infected with WT or ΔprgJ Salmonella Typhimurium or mock infected at 60 min post infection.2025-08-28T00:00:00Z2025-08-28T12:25:49.692Z2025-08-28T09:49:06.748ZE-MTAB-15534ERP179251EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184