biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsJan Kubovčiaktranscription profiling by arrayRattus norvegicusRattus norvegicushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15554We examined whether overexpression of Nrf2 gene, key antioxidant defense regulator, improves adipose tissue metabolism in spontaneously hypertensive rats (SHR). To achieve that, we assessed differences in gene expression profiles between Nrf2 transgenic and nontransgenic individuals using hybridization array.biostudies-arrayexpressNucleic Acid Extraction - Total RNA was extracted using using RNeasy Micro Kit (QIAGEN Sciences Inc, Germantown, MD, USA).Sample Treatment - Transgenic SHR line (hereafter referred to as the SHR-Nrf2) was derived by microinjections of fertilized eggs with the mix of Sleeping Beauty (SB) construct containing mouse Nrf2 cDNA under control of the SV universal promoter and mRNA of the SB100X transposase. Genotyping of positive rats was done by PCRHybridization - Affymetrix GeneChip® Rat Gene 1.0 ST Array System was used for the microarray analysis following the standard protocol.Sample Collection - After animals were killed, epididymal fat was collected and frozen until analysis.Scaning - Data were preprocessed in Partek Genomic Suit (Partek Incorporated)Labeling - 100 ng RNA was amplified with Ambion WT Expression Kit (Applied Biosystems), 5.5 μg single-stranded cDNA was labeled and fragmented with GeneChip WT Terminal Labeling and Hybridization (Affymetrix).MIAME ScoreRaw DataOrganizationAssays and DataMAGE-TAB FilesArray DesignsJan KubovčiakMichal PravenecfalseComparison of white adipose tissue expression profiles of Nrf2 overexpressing individuals versus wild-type controls in spontaneously hypertensive ratsWe examined whether overexpression of Nrf2 gene, key antioxidant defense regulator, improves adipose tissue metabolism in spontaneously hypertensive rats (SHR). To achieve that, we assessed differences in gene expression profiles between Nrf2 transgenic and nontransgenic individuals using hybridization array.2025-09-09T00:00:00Z2025-09-09T07:57:47.146Z2025-09-05T13:47:41.826ZE-MTAB-15554EFO_0002768EFO_0002944EFO_0003814EFO_0003813EFO_0005518EFO_0003815EFO_0003969