biostudies-arrayexpressMatteo ZanovelloHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15564We performed RNA-seq on HeLa cells to assess the shared transcriptomics changes between USP10 and TDP-43 overexpression, as well as to understand the effect of USP10 overexpression on TDP-43 loss-of-function targets.biostudies-arrayexpressLibrary Construction - KAPA polyASample Treatment - OE using viral vectors, KD using siRNASample Collection - scraping from plateSequencing - Illumina-seqNucleic Acid Extraction - RNeasy mini (QIAgen)Growth Protocol - cell culture for 10 days, changing media every 72 hoursOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesData Transformation - Salmon + DESeq2Sequence Alignment - STARUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAHomo sapiensMatteo ZanovellofalseRNA-seq from USP10 and TDP-43 overexpression and TDP-43 knockdown associated with or without USP10 overexpression in HeLa cellsWe performed RNA-seq on HeLa cells to assess the shared transcriptomics changes between USP10 and TDP-43 overexpression, as well as to understand the effect of USP10 overexpression on TDP-43 loss-of-function targets.2025-09-21T00:00:00Z2025-09-21T01:04:17.948Z2025-09-08T11:16:51.587ZE-MTAB-15564ERP179764EFO_0002944EFO_0004170EFO_0003789EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184EFO_0003969