biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsLars GrønvoldIllumina HiSeq 2500RNA-seq of coding RNAMucor circinelloidesMucor circinelloideshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15566The filamentous fungus Mucor circinelloides VI04473 (Cir2 in sample name) was grown to investigate the transcriptomic response to calcium availability under reference phosphate conditions. Cultures were grown in nitrogen-limited medium containing inorganic phosphate at the reference concentration P1 (7 g/L KH₂PO₄ and 2 g/L Na₂HPO₄), with two calcium treatments: calcium present at 0.1 g/L CaCl₂·2H₂O (Ca1) and calcium missing (Ca0). Biomass was harvested after 5 days of cultivation (4 biological replicates per treatment), RNA was extracted, and transcriptomes were sequenced for differential expression analysis between the calcium treatments.biostudies-arrayexpressLibrary Construction - Library preparation consisted of rRNA removal (polyA capture), followed by enrichment of single-stranded messenger RNAs (mRNAs) and reverse transcription to obtain complementary DNA (cDNA).Sample Collection - Fungal mass was cultivated and collected according to Dzurendova et al. (2021), section 2.2: Dzurendova, S., Zimmermann, B., Kohler, A., Reitzel, K., Nielsen, U. G., Dupuy--Galet, B. X., . . . Shapaval, V. (2021). Calcium Affects Polyphosphate and Lipid Accumulation in Mucoromycota Fungi. Journal of Fungi, 7(4), 300. https://doi.org/10.3390/jof7040300Sequencing - Illumina PE150 technology (paired-end 150 basepair sequencing strategy, short read), was utilized for sequencing the cDNA.Nucleic Acid Extraction - RNA extraction was carried out using the RNeasy Plus Mini kit (Qiagen, Venlo, The Netherlands).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesLars GrønvoldHelle Tessand BaalsrudfalseRNA-seq of Mucor circinelloides strain VI04473 grown with and without calciumThe filamentous fungus Mucor circinelloides VI04473 (Cir2 in sample name) was grown to investigate the transcriptomic response to calcium availability under reference phosphate conditions. Cultures were grown in nitrogen-limited medium containing inorganic phosphate at the reference concentration P1 (7 g/L KH₂PO₄ and 2 g/L Na₂HPO₄), with two calcium treatments: calcium present at 0.1 g/L CaCl₂·2H₂O (Ca1) and calcium missing (Ca0). Biomass was harvested after 5 days of cultivation (4 biological replicates per treatment), RNA was extracted, and transcriptomes were sequenced for differential expression analysis between the calcium treatments.2025-08-26T00:00:00Z2025-09-12T08:25:33.444Z2025-09-08T23:50:23.41ZE-MTAB-15566ERP179789E-MTAB-13309EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184