{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Dussaud sébastien"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15568"],"description":["Ulcerative colitis impact on Kupffer cell homeostasis was assessed using transcriptional analysis.  Kupffer cells were sorted from the livers of mice treated with dextran sodium sulphate for 7 days. Total RNA preparation was performed on 20,000 sorted Kupffer cells."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - cDNA libraries were generated using the KAPA mRNA HyperPrep kit (Roche).","Nucleic Acid Extraction - RNAs from sorted cells were prepared using the RNeasy Plus micro kit (Qiagen).","Sequencing - RNA-Seq libraries were sequenced on an Illumina NovaSeq 6000.","Sample Collection - Mouse liver tissue was harvested and digested in Hanks Balanced Salt Solution (HBSS,no calcium and magnesium) containing fetal bovine serum (FBS,1%) and collagenase D (2.5 mg/ml,Sigma-Aldrich) for 30 minutes at 37°C under agitation. After homogenization, the suspension is washed in PBS buffer containing bovine serum albumin (1%) and EDTA (0.5%). After centrifugation, liver cells are resuspended in Hanks Balanced Salt Solution. Cell suspensions were stained with appropriate antibodies for 30 min on ice. Kupffer cells sorting was performed on a BD FACSAria II™ cell sorter."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Dussaud sébastien"],"additional_accession":[]},"is_claimable":false,"name":"RNAseq of sorted Kupffer cells during ulcerative colitis","description":"Ulcerative colitis impact on Kupffer cell homeostasis was assessed using transcriptional analysis.  Kupffer cells were sorted from the livers of mice treated with dextran sodium sulphate for 7 days. Total RNA preparation was performed on 20,000 sorted Kupffer cells.","dates":{"release":"2026-09-02T00:00:00Z","modification":"2026-09-02T01:00:39.822Z","creation":"2025-09-12T10:31:41.707Z"},"accession":"E-MTAB-15568","cross_references":{"ENA":["ERP179972"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}