biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsDussaud sébastienIllumina NovaSeq 6000RNA-seq of coding RNAMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15568Ulcerative colitis impact on Kupffer cell homeostasis was assessed using transcriptional analysis. Kupffer cells were sorted from the livers of mice treated with dextran sodium sulphate for 7 days. Total RNA preparation was performed on 20,000 sorted Kupffer cells.biostudies-arrayexpressLibrary Construction - cDNA libraries were generated using the KAPA mRNA HyperPrep kit (Roche).Nucleic Acid Extraction - RNAs from sorted cells were prepared using the RNeasy Plus micro kit (Qiagen).Sequencing - RNA-Seq libraries were sequenced on an Illumina NovaSeq 6000.Sample Collection - Mouse liver tissue was harvested and digested in Hanks Balanced Salt Solution (HBSS,no calcium and magnesium) containing fetal bovine serum (FBS,1%) and collagenase D (2.5 mg/ml,Sigma-Aldrich) for 30 minutes at 37°C under agitation. After homogenization, the suspension is washed in PBS buffer containing bovine serum albumin (1%) and EDTA (0.5%). After centrifugation, liver cells are resuspended in Hanks Balanced Salt Solution. Cell suspensions were stained with appropriate antibodies for 30 min on ice. Kupffer cells sorting was performed on a BD FACSAria II™ cell sorter.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesDussaud sébastienfalseRNAseq of sorted Kupffer cells during ulcerative colitisUlcerative colitis impact on Kupffer cell homeostasis was assessed using transcriptional analysis. Kupffer cells were sorted from the livers of mice treated with dextran sodium sulphate for 7 days. Total RNA preparation was performed on 20,000 sorted Kupffer cells.2026-09-02T00:00:00Z2026-09-02T01:00:39.822Z2025-09-12T10:31:41.707ZE-MTAB-15568ERP179972EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184