{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Lisa DUGAST"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15576"],"description":["Freshly isolated PBMCs from three healthy volunteers were incubated with a depleting antibody or its isotype control for several hours before being analysed by CITEseq and single cell RNAseq."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - Single cell libraries were performed according to Chromium Next GEM Single Cell 5' Reagent Kits v2 protocol (10X genomics, San Francisco, CA). For each sample, cells were processed in one of eight wells and multiplexed using antibody hashtags","Sequencing - Two libraries were prepared for mRNA (RNA) and for barcoded antibodies (HTO and CITE-seq). They were sequenced with NovaSeq 6000 (Illumina, San Diego, CA) according to 10X genomics recommendations","Nucleic Acid Extraction - Disintegration of cells or tissues (cell lysis), inactivation of intracellular nucleases, and separation of nucleic acids from the other cell components","Sample Collection - Freshly isolated PBMCs of three healthy donors were labelled with anti-human Hashtag antibodies TotalSeq-C (Biolegend, San Diego, CA) and were pooled together"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - FASTQ files generated from BaseCalling (BCL) files with CellRanger package (6.1.2) were demultiplexed and aligned to human reference genome (hg38). CITE-seq-Count function (1.3.4) was used to count antibody hashtag sequences"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA from single cells"],"species":["Homo sapiens"],"pubmed_authors":["Lisa DUGAST","Carole GUILLONNEAU"],"additional_accession":[]},"is_claimable":false,"name":"Anti-CD45RC antibody targets CD45RChigh T and B cells to mitigate pathogenic immune responses","description":"Freshly isolated PBMCs from three healthy volunteers were incubated with a depleting antibody or its isotype control for several hours before being analysed by CITEseq and single cell RNAseq.","dates":{"release":"2025-11-20T00:00:00Z","modification":"2026-05-27T16:04:22.727Z","creation":"2025-09-12T14:31:34.392Z"},"accession":"E-MTAB-15576","cross_references":{"ENA":["ERP180027"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005684","EFO_0005518","EFO_0003816","EFO_0004184"]}}