biostudies-arrayexpressYue YuanDanio reriohttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15577Bulk RNA-Seq of muscle isolated from zebrafish carrying a mutation in telomerase (tert) and wildtype sibling animals aged 5 days at 24 hpf following needle stick injury. RNA was extracted from pooled samples of n=50 larvae each on 30/01/2024. Libraries were constructed using Illumina TruSeq RNA Library Prep Kit and sequenced on an Illumina NovaSeq 6000 with paired end reads of 150 bp generated by Novogene.Result come back on 07.03.2024.biostudies-arrayexpressSample Collection - All RNA analyses were performed using triplicates for all conditions using material isolated from the muscle of 30 larvae using Tri-reagent.Library Construction - mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP for non-directional library.Nucleic Acid Extraction - Total RNA was isolated from dissected injured trunk regions of larvae at 24h post injury (hpi). Tissue was homogenised in TriReagent (Sigma) and total RNA isolated according to the manufacturer’s protocol.Sequencing - The sequencing was performed by Novogene (UK) Company Limited, using Illumina NovaSeq Platforms With Paired-end 150bp Sequencing StrategyOrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Index of the reference genome was built using Hisat2 v2.0.5 and paired-end clean reads were aligned to the reference genome Danio_rerio.GRCz11.dna using Hisat2 v2.0.5MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XRNA-seq of coding RNADanio rerioMuscle regeneration can be rescued in a telomerase deficient zebrafish model of ageing by MMP inhibitionYue YuanYue Yuan, Carlene Dyer, Robert KnightfalseRNA-seq of 5dpf zebrafish tert F2 larvae with/without injuryBulk RNA-Seq of muscle isolated from zebrafish carrying a mutation in telomerase (tert) and wildtype sibling animals aged 5 days at 24 hpf following needle stick injury. RNA was extracted from pooled samples of n=50 larvae each on 30/01/2024. Libraries were constructed using Illumina TruSeq RNA Library Prep Kit and sequenced on an Illumina NovaSeq 6000 with paired end reads of 150 bp generated by Novogene.Result come back on 07.03.2024.2025-09-25T00:00:00Z2026-05-27T18:12:19.311Z2025-09-12T11:42:30.325ZE-MTAB-15577ERP180012EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184