biostudies-arrayexpressHamidah RaduwanMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15581Examination of the transcriptional changes occurring in the skin upon mosquito bite at different times of day. Approximately 20 A. aegypti mosquitoes were allowed to bite mice on the ear at two timepoints, zeitgeber time (ZT)1 and ZT11, corresponding to dawn and dusk, respectively. Twenty-four hours later, biopsies from the mosquito bite sites were collected and transcriptomic analyses were performed. Biopsies from mice that did not receive a mosquito bite served as controlsbiostudies-arrayexpressSample Collection - The mosquito bite sites were then marked with surgical skin marker. 24 hours later, 3.5mm ear punch biopsies were taken. Tissues are collected into RLT buffer (Qiagen Cat#79216) and immediately subjected to RNA extraction as described below.Nucleic Acid Extraction - RNA extraction was performed from murine skin using the RNeasy fibrous tissue mini kit (Qiagen 74704) following the manufacturer’s protocol. Briefly, skin tissues were homogenized using a KIMBLE pestle inside a centrifuge tube for 30s-1min or until most tissues were lysed. Proteinase K was then added, and tissues were incubated in a 55C water bath for 10min. The mixture was spun at 10,000g for 5min and supernatant collected in a new tube. Ethanol was added to the supernatant, gently pipetted for mixing, and transferred to an RNeasy Mini column. Columns were spun to allow RNA to bind to the silica, washed with RW1, RDD, and RPE buffer per manufacturer’s protocol. Samples were eluted with 30ul RNAse-free water and subjected to RNA-sequencing.Sequencing - Sequencing was done on Illumina HiSeq 2500 instrument by generating paired end reads at 150 bp length at the Yale Center for Genome Analysis (YCGA).Library Construction - RNA libraries were prepared by removal of ribosomal RNAOrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - The gene-level data were normalized using likelihood ratio test in edgeR (v4.2.1).UnknownTranscriptomicsGenomicsProteomicsIllumina HiSeq 2500RNA-seq of total RNAMus musculusHamidah RaduwanfalseThe cutaneous response to a mosquito bite at different diurnal time pointExamination of the transcriptional changes occurring in the skin upon mosquito bite at different times of day. Approximately 20 A. aegypti mosquitoes were allowed to bite mice on the ear at two timepoints, zeitgeber time (ZT)1 and ZT11, corresponding to dawn and dusk, respectively. Twenty-four hours later, biopsies from the mosquito bite sites were collected and transcriptomic analyses were performed. Biopsies from mice that did not receive a mosquito bite served as controls2025-09-16T00:00:00Z2025-09-16T01:02:19.694Z2025-09-08T13:04:44.264ZE-MTAB-15581ERP179772EFO_0002944EFO_0004170EFO_0009653EFO_0005518EFO_0003816EFO_0004184