biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsSeungwan WooTru-cut biopsy needle (Marquee®, 16 G × 10 cm)Illumina NovaSeq XTruSeq Stranded Total RNA Library Prep Gold Kit, TruSeq Stranded Total RNA Globin Kit.RNA-seq of coding RNACanis lupus familiarisCanis lupus familiarishttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15590Canine oral melanoma is a highly aggressive tumor with limited therapeutic options and a poor prognosis. To elucidate the molecular mechanisms underlying its progression and response to radiation therapy (RT), we performed a longitudinal multi-omics analysis incorporating whole-genome sequencing (WGS) and RNA sequencing (RNA-seq) on tumor and blood samples from a single canine case. WGS was performed on tumor and blood collected before RT (Pre-RT) and on blood collected immediately after the final radiation fraction (Post-RT 0m). RNA-seq was performed on tumor and blood collected before RT (Pre-RT) and immediately after the final radiation fraction (Post-RT 0m), and on tumor collected 4 months after RT (Post-RT 4m).biostudies-arrayexpressSample Collection - Tumor tissue was collected by Tru-cut biopsy and preserved in RNAlater™ at three time points: before RT (Pre-RT), immediately after the final radiation fraction (Post-RT 0 m), and 4 months post-RT (Post-RT 4 m). Peripheral blood was drawn from the external jugular vein into Tempus™ Blood RNA Tubes at two time points: before RT (Pre-RT) and immediately after the final radiation fraction (Post-RT 0m). All samples were stored at −80 °C until processing. Animal procedures were approved by IACUC (GNU-240826-D0172).Nucleic Acid Extraction - Total RNA was extracted from tumor (Pre-RT, Post-RT 0 m, Post-RT 4 m) and blood (Pre-RT, Post-RT 0 m) by the service provider according to internal standard operating procedures.Library Construction - Libraries were prepared using TruSeq Stranded Total RNA Library Prep Gold Kit (Illumina, San Diego, CA, USA) for tumor samples and TruSeq Stranded Total RNA Globin Kit (Illumina, San Diego, CA, USA) for blood samples.Sequencing - Paired-end sequencing was performed on an Illumina NovaSeq X platform (Illumina, San Diego, CA, USA).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesSeungwan WoofalseRNA sequencing of canine oral melanoma and matched peripheral blood pre- and post-radiotherapyCanine oral melanoma is a highly aggressive tumor with limited therapeutic options and a poor prognosis. To elucidate the molecular mechanisms underlying its progression and response to radiation therapy (RT), we performed a longitudinal multi-omics analysis incorporating whole-genome sequencing (WGS) and RNA sequencing (RNA-seq) on tumor and blood samples from a single canine case. WGS was performed on tumor and blood collected before RT (Pre-RT) and on blood collected immediately after the final radiation fraction (Post-RT 0m). RNA-seq was performed on tumor and blood collected before RT (Pre-RT) and immediately after the final radiation fraction (Post-RT 0m), and on tumor collected 4 months after RT (Post-RT 4m).2025-09-15T00:00:00Z2025-09-15T23:11:29.061Z2025-09-12T12:41:17.324ZE-MTAB-15590ERP180018EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184