biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsSeungwan WooTru-cut biopsy needle (Marquee®, 16 G × 10 cm)Illumina NovaSeq X.TruSeq PCR-free Sample Preparation KitDNA-seqCanis lupus familiarisCanis lupus familiarishttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15592Canine oral melanoma is a highly aggressive tumor with limited therapeutic options and a poor prognosis. To elucidate the molecular mechanisms underlying its progression and response to radiation therapy (RT), we performed a longitudinal multi-omics analysis incorporating whole-genome sequencing (WGS) and RNA sequencing (RNA-seq) on tumor and blood samples from a single canine case. WGS was performed on tumor and blood collected before RT (Pre-RT) and on blood collected immediately after the final radiation fraction (Post-RT 0m). RNA-seq was performed on tumor and blood collected before RT (Pre-RT) and immediately after the final radiation fraction (Post-RT 0m), and on tumor collected 4 months after RT (Post-RT 4m).biostudies-arrayexpressLibrary Construction - PCR-free whole-genome libraries were prepared using the TruSeq PCR-free Sample Preparation Kit (Illumina). Library QC was performed with KAPA Library Quantification and Agilent High Sensitivity DNA chip.Sample Collection - Tumor tissue was obtained by Tru-cut biopsy and preserved in RNAlater™ before RT. Peripheral blood was drawn from the external jugular vein into Tempus™ Blood RNA Tubes at two time points: before RT (Pre-RT) and immediately after the final radiation fraction (Post-RT 0m). Samples were stored at −80 °C until processing. Animal procedures were approved by the IACUC (GNU-240826-D0172).Nucleic Acid Extraction - Genomic DNA was extracted from Pre-RT Tumor, Pre-RT Blood, and Post-RT 0 m Blood by the service provider following internal standard operating procedures.Sequencing - Paired-end whole-genome sequencing was performed on an Illumina NovaSeq X platform (Illumina, San Diego, CA, USA).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesSeungwan WoofalseWhole-genome sequencing of canine oral melanoma and matched peripheral blood pre- and post-radiotherapyCanine oral melanoma is a highly aggressive tumor with limited therapeutic options and a poor prognosis. To elucidate the molecular mechanisms underlying its progression and response to radiation therapy (RT), we performed a longitudinal multi-omics analysis incorporating whole-genome sequencing (WGS) and RNA sequencing (RNA-seq) on tumor and blood samples from a single canine case. WGS was performed on tumor and blood collected before RT (Pre-RT) and on blood collected immediately after the final radiation fraction (Post-RT 0m). RNA-seq was performed on tumor and blood collected before RT (Pre-RT) and immediately after the final radiation fraction (Post-RT 0m), and on tumor collected 4 months after RT (Post-RT 4m).2025-09-16T00:00:00Z2025-09-16T01:02:17.684Z2025-09-15T11:23:52.575ZE-MTAB-15592ERP180056EFO_0002944EFO_0004170EFO_0002693EFO_0005518EFO_0004184