{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Martin Alcaraz Jr."],"instrument_platform":["Illumina NovaSeq X"],"study_type":["RNA-seq of coding RNA"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15597"],"description":["12-week old B6 Kras-LUAD mice were maintained on a control diet or alpha-ketoglutarate diet for 16-weeks before LUAD tumors were collected and used for bulk RNA-sequencing."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Total RNA was isolated from murine LUAD tumors using the Qiagen RNeasy Mini Kit (cat. #74104) following the manufacturer’s instructions. RNA integrity and concentration were assessed with the Agilent 2100 Bioanalyzer.","Library Construction - Libraries for RNA-Seq were prepared using the KAPA Stranded mRNA-Seq Kit (Roche). The workflow included mRNA enrichment and fragmentation, first strand cDNA synthesis using random priming, and second strand synthesis incorporating dUTP. Double-stranded cDNA was end-repaired, A-tailed, ligated to sequencing adapters, and PCR-amplified. Unique adapters were used for multiplexing samples.","Sequencing - Sequencing was performed on an Illumina NovaSeq X Plus platform with paired-end 2 × 50 bp reads. Data quality was assessed with Illumina SAV, and demultiplexing was carried out using Illumina bcl2fastq v2.19.1.403.","Sample Collection - Murine LUAD tumors were collected at 16-weeks post treatment (diet) and were immediately snapfrozen in liquid nitrogen and placed in a -80 freezer until RNA extraction."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Martin Alcaraz Jr.","Claudio Scafoglio"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of murine LUAD tumors on control diet or alpha-ketoglutarate diet for 16-weeks compared by sex","description":"12-week old B6 Kras-LUAD mice were maintained on a control diet or alpha-ketoglutarate diet for 16-weeks before LUAD tumors were collected and used for bulk RNA-sequencing.","dates":{"release":"2026-09-01T00:00:00Z","modification":"2026-09-01T07:08:02.508Z","creation":"2025-09-12T13:25:34.71Z"},"accession":"E-MTAB-15597","cross_references":{"ENA":["ERP180023"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}