biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsMartin Alcaraz Jr.Illumina NovaSeq XRNA-seq of coding RNAMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-1559712-week old B6 Kras-LUAD mice were maintained on a control diet or alpha-ketoglutarate diet for 16-weeks before LUAD tumors were collected and used for bulk RNA-sequencing.biostudies-arrayexpressNucleic Acid Extraction - Total RNA was isolated from murine LUAD tumors using the Qiagen RNeasy Mini Kit (cat. #74104) following the manufacturer’s instructions. RNA integrity and concentration were assessed with the Agilent 2100 Bioanalyzer.Library Construction - Libraries for RNA-Seq were prepared using the KAPA Stranded mRNA-Seq Kit (Roche). The workflow included mRNA enrichment and fragmentation, first strand cDNA synthesis using random priming, and second strand synthesis incorporating dUTP. Double-stranded cDNA was end-repaired, A-tailed, ligated to sequencing adapters, and PCR-amplified. Unique adapters were used for multiplexing samples.Sequencing - Sequencing was performed on an Illumina NovaSeq X Plus platform with paired-end 2 × 50 bp reads. Data quality was assessed with Illumina SAV, and demultiplexing was carried out using Illumina bcl2fastq v2.19.1.403.Sample Collection - Murine LUAD tumors were collected at 16-weeks post treatment (diet) and were immediately snapfrozen in liquid nitrogen and placed in a -80 freezer until RNA extraction.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMartin Alcaraz Jr.Claudio ScafogliofalseRNA-seq of murine LUAD tumors on control diet or alpha-ketoglutarate diet for 16-weeks compared by sex12-week old B6 Kras-LUAD mice were maintained on a control diet or alpha-ketoglutarate diet for 16-weeks before LUAD tumors were collected and used for bulk RNA-sequencing.2026-09-01T00:00:00Z2026-09-01T07:08:02.508Z2025-09-12T13:25:34.71ZE-MTAB-15597ERP180023EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184