{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Brian Hendrich"],"instrument_platform":["Illumina NovaSeq X"],"study_type":["CUT&RUN"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15606"],"description":["This study investigates the role of ADNP in regulating CHD4 and MBD3 binding using CUT&RUN. CUT&RUN was performed to capture changes in the binding of CHD4 (using FLAG-tag) and MBD3. Two biological replicates were processed for each protein with two technical replicates. This dataset aims to characterise the binding of CHD4 and MBD3 in mouse ES cells."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - DNA was extracted using Zymo DNA Clean and Concentrator Kit-5 (Cat. No. D4014).","Sequencing - 300bp paired-end (2 x 150 bp) sequencing was performed on a NovaSeqX 25B flowcell at the Cambridge Stem Cell Institute and CRUK sequencing facilities.","Sample Collection - Cells were cultured on 10cm plates. Cells from all conditions were harvested at the same time using accutase and spun down for 3 min, 600 x g in a swinging bucket rotor at room temperature. Cells were washed in PBS and processed immediately.","Growth Protocol - ES cells were cultured on 0.1% gelatin-coated plates in 2i/LIF conditions.","Library Construction - Library preparation for sequencing was carried out in the SCI facilities."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Brian Hendrich","Andria Koulle"],"additional_accession":[]},"is_claimable":false,"name":"CUT&RUN data of Chd4 and Mbd3 in mouse embryonic stem cells","description":"This study investigates the role of ADNP in regulating CHD4 and MBD3 binding using CUT&RUN. CUT&RUN was performed to capture changes in the binding of CHD4 (using FLAG-tag) and MBD3. Two biological replicates were processed for each protein with two technical replicates. This dataset aims to characterise the binding of CHD4 and MBD3 in mouse ES cells.","dates":{"release":"2026-01-15T00:00:00Z","modification":"2026-01-15T12:58:05.979Z","creation":"2025-09-18T17:31:46.304Z"},"accession":"E-MTAB-15606","cross_references":{"ENA":["ERP180287"],"EFO":["EFO_0002944","EFO_0009973","EFO_0004170","EFO_0003789","EFO_0005518","EFO_0004184"]}}