biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsBrian HendrichIllumina NovaSeq XCUT&RUNMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15606This study investigates the role of ADNP in regulating CHD4 and MBD3 binding using CUT&RUN. CUT&RUN was performed to capture changes in the binding of CHD4 (using FLAG-tag) and MBD3. Two biological replicates were processed for each protein with two technical replicates. This dataset aims to characterise the binding of CHD4 and MBD3 in mouse ES cells.biostudies-arrayexpressNucleic Acid Extraction - DNA was extracted using Zymo DNA Clean and Concentrator Kit-5 (Cat. No. D4014).Sequencing - 300bp paired-end (2 x 150 bp) sequencing was performed on a NovaSeqX 25B flowcell at the Cambridge Stem Cell Institute and CRUK sequencing facilities.Sample Collection - Cells were cultured on 10cm plates. Cells from all conditions were harvested at the same time using accutase and spun down for 3 min, 600 x g in a swinging bucket rotor at room temperature. Cells were washed in PBS and processed immediately.Growth Protocol - ES cells were cultured on 0.1% gelatin-coated plates in 2i/LIF conditions.Library Construction - Library preparation for sequencing was carried out in the SCI facilities.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesBrian HendrichAndria KoullefalseCUT&RUN data of Chd4 and Mbd3 in mouse embryonic stem cellsThis study investigates the role of ADNP in regulating CHD4 and MBD3 binding using CUT&RUN. CUT&RUN was performed to capture changes in the binding of CHD4 (using FLAG-tag) and MBD3. Two biological replicates were processed for each protein with two technical replicates. This dataset aims to characterise the binding of CHD4 and MBD3 in mouse ES cells.2026-01-15T00:00:00Z2026-01-15T12:58:05.979Z2025-09-18T17:31:46.304ZE-MTAB-15606ERP180287EFO_0002944EFO_0009973EFO_0004170EFO_0003789EFO_0005518EFO_0004184