{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Richard Cubbon"],"instrument_platform":["NextSeq 500"],"study_type":["RNA-seq of coding RNA"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15624"],"description":["Bone marrow derived macrophages (BMDMs) were cultured from hind limb bone marrow (femur plus tibia) collected from C57Bl/6 mice 7-days after induction of unilateral (left) hind limb ischaemia by ligation of the femoral artery, along with control mice not undergoing surgery, providing 6 control mouse samples, 3 ischaemic limb samples and 3 non-ischaemic limb samples. Further BMDMs from these groups were also cultured with lipopolysaccharide (LPS) to explore the impact of bone marrow ischaemia on a pathogen-associated molecular pattern response, providing 6 control mouse LPS samples, 3 ischaemic limb LPS samples and 3 non-ischaemic limb LPS samples."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - TruSeq Stranded mRNA libraries made from murine total RNA","Sample Collection - Total RNA was isolated from naïve or LPS-stimulated BMDMs after 7 days of differentiation using TRI Reagent (Sigma-Aldrich)","Nucleic Acid Extraction - RNA Clean & Concentrator-5 kit (Zymo: R1012) with DNase I treatment.","Sequencing - NextSeq 500, 75 bp single end lane"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Richard Cubbon"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of bone marrow derived macrophages from mice undergoing hind-limb ischaemia surgery versus controls with or without lipopolysaccharide stimulation","description":"Bone marrow derived macrophages (BMDMs) were cultured from hind limb bone marrow (femur plus tibia) collected from C57Bl/6 mice 7-days after induction of unilateral (left) hind limb ischaemia by ligation of the femoral artery, along with control mice not undergoing surgery, providing 6 control mouse samples, 3 ischaemic limb samples and 3 non-ischaemic limb samples. Further BMDMs from these groups were also cultured with lipopolysaccharide (LPS) to explore the impact of bone marrow ischaemia on a pathogen-associated molecular pattern response, providing 6 control mouse LPS samples, 3 ischaemic limb LPS samples and 3 non-ischaemic limb LPS samples.","dates":{"release":"2026-09-14T00:00:00Z","modification":"2026-09-17T10:39:14.241Z","creation":"2025-09-23T12:03:30.541Z"},"accession":"E-MTAB-15624","cross_references":{"ENA":["ERP180514"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}