biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsRodrigo Arcoverde Cerveiratranscription profiling by arrayMacaca mulattaMacaca mulattahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15632Whole blood was collected from cynomolgus macaques into PAXgene Blood RNA tubes (PreAnalytiX) and stored at –20 °C until RNA extraction. Animals were divided into three vaccine groups: mRNA influenza vaccine (n = 5), Vaxigrip (n = 4), and Fluad (n = 5). Samples were taken at baseline (0 h), 24 h post-vaccination, and for some animals at 24 h after a booster, resulting in 35 samples in total. RNA was extracted using Trizol reagent (Ambion) with Tissuelyser (QIAGEN) disruption, followed by labeling with the Quick Amp Labeling Kit (Agilent) and purification with RNeasy spin columns (QIAGEN). Labeled cRNA was hybridized to Agilent Rhesus Macaque Gene Expression Microarrays v2 (G2519F-026806), scanned on an Agilent DNA Microarray Scanner (G2505C), and processed with Agilent Feature Extraction Software.biostudies-arrayexpressHybridization - Cy3-labeled cRNA was hybridized to Agilent Rhesus Macaque Gene Expression Microarrays v2 (part number G2519F-026806) for 17 h at 65 °C, according to the manufacturer’s instructions.Labeling - Cyanine-3 (Cy3)-labeled cRNA was synthesized from 200 ng total RNA with the Quick Amp Labeling Kit (Agilent) according to the manufacturer’s protocol, followed by purification on RNeasy spin columns.Scaning - Slides were scanned using the Agilent DNA Microarray Scanner (G2505C). Feature extraction and signal processing were performed with Agilent Feature Extraction Software.Sample Collection - Whole blood was collected from cynomolgus macaques into PAXgene Blood RNA tubes (PreAnalytiX) at baseline (0 h), 24 h post-vaccination, and 24 h post-boost for some animals. Tubes were stored at –20 °C until RNA extraction.Nucleic Acid Extraction - Total RNA was extracted from whole blood using Trizol reagent (Ambion) with disruption on a Tissuelyser (QIAGEN), following manufacturer’s instructions. RNA was purified using RNeasy spin columns (QIAGEN).MIAME ScoreRaw DataOrganizationAssays and DataProcessed DataorganisationMAGE-TAB FilesArray DesignsRodrigo Arcoverde CerveiraKarin LoréData Transformation - Raw array intensities (gProcessedSignal) were obtained from Agilent Feature Extraction Software. Quantile normalization of gProcessed Signals.falseBlood Microarray of rhesus macaque following flu vaccinationWhole blood was collected from cynomolgus macaques into PAXgene Blood RNA tubes (PreAnalytiX) and stored at –20 °C until RNA extraction. Animals were divided into three vaccine groups: mRNA influenza vaccine (n = 5), Vaxigrip (n = 4), and Fluad (n = 5). Samples were taken at baseline (0 h), 24 h post-vaccination, and for some animals at 24 h after a booster, resulting in 35 samples in total. RNA was extracted using Trizol reagent (Ambion) with Tissuelyser (QIAGEN) disruption, followed by labeling with the Quick Amp Labeling Kit (Agilent) and purification with RNeasy spin columns (QIAGEN). Labeled cRNA was hybridized to Agilent Rhesus Macaque Gene Expression Microarrays v2 (G2519F-026806), scanned on an Agilent DNA Microarray Scanner (G2505C), and processed with Agilent Feature Extraction Software.2026-04-21T00:00:00Z2026-06-07T17:42:48.039Z2025-09-24T14:29:51.691ZE-MTAB-15632EFO_0002768EFO_0002944EFO_0003814EFO_0003813EFO_0005518EFO_0003816EFO_0003815