biostudies-arrayexpressBen WangHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15670We performed the standard surgical procedure of total hysterectomy, bilateral adnexectomy and pelvic lymph node dissection on patients with early-stage endometrial cancer. A 72-month follow-up observation was conducted after the surgery to monitor the recurrence situation.The samples is T1aN0M0; Three samples (B80203,C19284,C17025) is not recurrent, one sample (C13956) is recurrent after 12 months.biostudies-arrayexpressLibrary Construction - We used Single-cell 30 Library and Gel Bead Kit V3 (10x Genomics) for cell capture and cDNA synthesis. The cells were counted using Count Star and resuspended. Cell suspensions were loaded on the 10x Genomics scRNA-seq platform to generate single-cell gel beads in the emulsion (GEM). The mRNA of single cells was reverse transcribed to produce cDNA, and the individual GEMs were simultaneously barcoded, which were programmed at 53°C for 45 min, 85°C for 5 min, and held at 4°C. The cDNA library was then generated, amplified, and assessed for quality using the Agilent 4200. Sequencing was performed.Sequencing - Read preprocessing was performed using the 10x Genomics workflow. Briefly, FASTQ files were processed by the function ‘CellRanger count’ of software CellRanger (10x Genomics, Version 4.0.0) for demultiplexing, barcode assignment, and quantification of unique molecular identifiers. Using a prebuilt annotation package, we mapped the reads to the mouse reference genome to generate gene expression matrices. The matrix files arising from different batches of experiments were subsequently analyzed using Seurat v3 software.Sample Collection - early endometrial adenocarcinoma of human (lesions not extending beyond the uterus, FIGO 2009 stage I and II, all patients underwent standard endometrial cancer surgery (total hysterectomy + bilateral adnexectomy + pelvic lymph node dissection ± abdominal main lymph node dissection)Nucleic Acid Extraction - we added Hibernate-A buffer containing 10% fetal bovine serum to terminate the digestion and gently pipetted the tissues multiple times for dissociation. The dissociated cells were filtered through a 40 mm cell strainer to remove cell aggregates and ultimately obtain a single-cell suspension for encapsulation with 10x Genomics.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Read alignment and transcript quantification were conducted using cellRanger v4.0.0MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina HiSeq 4000single nucleus RNA sequencingHomo sapiensBen WangfalseRecurrent early-stage endometrial cancer and non-recurrent early-stage endometrial cancerWe performed the standard surgical procedure of total hysterectomy, bilateral adnexectomy and pelvic lymph node dissection on patients with early-stage endometrial cancer. A 72-month follow-up observation was conducted after the surgery to monitor the recurrence situation.The samples is T1aN0M0; Three samples (B80203,C19284,C17025) is not recurrent, one sample (C13956) is recurrent after 12 months.2025-10-20T00:00:00Z2026-05-27T15:10:23.655Z2025-10-08T14:18:18.495ZE-MTAB-15670ERP181217EFO_0002944EFO_0004170EFO_0009809EFO_0005518EFO_0003816EFO_0004184