biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsZheng MinHiSeq X TenRNA-seq of coding RNAEpinephelus fuscoguttatus x Epinephelus lanceolatusEpinephelus fuscoguttatus x Epinephelus lanceolatushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15691Vibrio harveyi is an important pathogen in aquaculture, which can cause severe intestinal damage and metabolic disorders in fish. The intestinal damage of hybrid grouper caused by Vibrio harveyi was verified through transcriptional analysis.biostudies-arrayexpressLibrary Construction - cDNA libraries were constructed as per the instruction manual of NEBNext Ultra RNA Library Prep Kit.Nucleic Acid Extraction - Total RNA was extracted from the liver of hybrid grouper by using the TRlzol Reagent and following the manufacturer’s instructions (Life Technologies, California, USA).Sequencing - These built cDNA libraries were sequenced on an Illumina Hi-Seq X Ten system by the OE Biotech Co., Ltd. (Shanghai, China).Sample Collection - Before the start of the experiment, 600 healthy hybrid groupers (with an average body weight of 42.3 ± 2.8 g) were acclimatized in a recirculating aquaculture system (water temperature: 28–30°C; pH: 7.8 ± 0.2) and fasted for 24 hours. Subsequently, the fish were randomly assigned to two treatment groups: the control group (con), which received no treatment; and the Vibrio harveyi-infected group (HW), which was administered Vibrio harveyi via oral gavage.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesZheng MinfalseThe impact of Vibrio harveyi infection on the intestine of hybrid grouper (Epinephelus fuscoguttatus × Epinephelus lanceolatus)Vibrio harveyi is an important pathogen in aquaculture, which can cause severe intestinal damage and metabolic disorders in fish. The intestinal damage of hybrid grouper caused by Vibrio harveyi was verified through transcriptional analysis.2025-10-25T00:00:00Z2025-10-25T01:02:35.72Z2025-10-10T12:36:24.183ZE-MTAB-15691ERP181351EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184