biostudies-arrayexpressGiulio AcetoHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15705RNA-seq samples in BIN-67, COV434, SCCOHT-1 cells including Control (Ctrl.) and A-485 treatment (24h, 1μM)biostudies-arrayexpressLibrary Construction - 1ug total RNA was used for the library construction. RNA libraries for RNA-Seq were prepared using the SMARTER mRNA-Seq library.Sample Collection - BIN-67, COV434, SCCOHT-1 cells were cultured in Roswell Park Memorial Institute 1640 Medium (RPMI, Thermo Fisher Scientific, Cat# 11875-093; no pyruvate) with 6% fetal bovine serum (Sigma, Cat# F1051), 1% penicillin/streptomycin (Thermo Fisher Scientific, Cat# 15140-122), and 2 mM L-glutamine (Thermo Fisher Scientific, Cat# 25030-081). Cells were maintained in a humidified 5% CO2-containing incubator at 37°C and regular Mycoplasma test was performed using Mycoalert Detection Kit (Lonza, Cat# LT07-318). Cells were treated with 1μM A-485 for 24h. Total RNA was collected with the RNeasy Mini Kit (Qiagen, Hilden, Germany).Sequencing - Samples were sequenced with a Next Seq 500 instrumentNucleic Acid Extraction - Total RNA was extracted with the RNeasy Mini Kit (Qiagen, Hilden, Germany).OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Sequencing files were aligned to the human reference genome (hg38) using STAR (v2.6.1c). Gene expression levels were quantified using Homer or HTseq with Gencode gene annotation (GTF) files. Differentially expressed genes were identified using Edger.UnknownTranscriptomicsGenomicsProteomicsNextSeq 500RNA-seq of coding RNAHomo sapiensGiulio AcetoSidong HuangfalseEP300 acetyltransferase inhibition in SCCOHT cell linesRNA-seq samples in BIN-67, COV434, SCCOHT-1 cells including Control (Ctrl.) and A-485 treatment (24h, 1μM)2026-05-05T00:00:00Z2026-05-05T18:00:08.037Z2025-10-09T21:38:40.245ZE-MTAB-15705ERP181311EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184