biostudies-arrayexpressSteffen MöllerHomo sapiensDRAGEN BCL Convert (https://support.illumina.com/sequencing/sequencing_software/bcl-convert/) and quality-checked using FastQC (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/).https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15709All p73 isoforms share an identical DNA-binding domain. These CUT&RUN datasets for TAp73α, DNp73β, and a GFP control illustrate the isoform-specific differences in genomic binding. As no antibodies are currently available that selectively recognize individual p73 variants, the data were generated following overexpression of the individual variant.biostudies-arrayexpressSequencing - Loading concentration of 750 pM on a NextSeq 1000/2000 system using a P1 flow cell according to manufacturer's instructions.Nucleic Acid Extraction - Immunoprecipitation was performed with p73 antibody (1:1000; Thermo Fisher Scientific, USA)Sample Collection - CUT&RUN assays were conducted on SK-Mel-29 melanoma cells transduced with Ad.TAp73α, Ad.DNp73β or the Ad.GFP control, following the manufacturer’s protocol (CUT&RUN Assay Kit, Cell Signaling, USA).Sample Treatment - Adenoviral vectors encoding TAp73α, DNp73β, or GFP have been reported previously (Stiewe et al. 2002; Buhlmann et al. 2008). Infections of SK-Mel-29 were performed at a multiplicity of infection selected to achieve 100% transduction of the target cells.Library Construction - libraries were prepared with the DNA Library Prep Kit for Illumina Systems (Cell Signaling, USA) according to the instructionsGrowth Protocol - Human SK-Mel-29 and SaOS-2 cell lines were maintained at 37 °C in a humidified atmosphere with 5% CO₂ in Dulbecco’s modified Eagle’s medium (DMEM; PAN-Biotech, Germany), containing pyruvate for SK-Mel-29 and pyruvate-free for SaOS-2. Media were supplemented with 10% fetal calf serum (FCS; PAN-Biotech, Germany), 0.1 mM non-essential amino acids, 50 U/mL penicillin, and 50 µg/mL streptomycin.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMetabolomicsUnknownTranscriptomicsGenomicsProteomicsNextSeq 2000CUT&RUNHomo sapiensTAp73α drives cancer metastasis via PPI-mediated derepression of the neuronal HDAC2/REST-GABBR2 axis.Integrative Multimodal Profiling of TAp73 and DNp73 Reveals Isoform-Specific Transcriptomic Coregulator Landscapes in Cancer Programs.Steffen MöllerNares TrakooljulMurr N, Richter C, Gupta SK, Hammer E, Trakooljul N, Stoll A, Möller S, Neumann LE, Pützer BM, Spitschak A.Möller S, Spitschak A, Murr N, Pützer BM.Nico MurrAlf SpitschakfalseBinding of full length p73 (TAp73alpha) and cancer-specific variant (DNp73beta) to genome of melanoma cell line SK-Mel-29All p73 isoforms share an identical DNA-binding domain. These CUT&RUN datasets for TAp73α, DNp73β, and a GFP control illustrate the isoform-specific differences in genomic binding. As no antibodies are currently available that selectively recognize individual p73 variants, the data were generated following overexpression of the individual variant.2026-03-30T00:00:00Z2026-04-21T15:09:55.286Z2025-10-13T15:57:31.398ZE-MTAB-15709publ-0-mohi-removablepubl-0-02hq-removableERP182066E-MTAB-14704EFO_0002944EFO_0009973EFO_0004170EFO_0003789EFO_0005518EFO_0004184EFO_000396910.1016/j.canlet.2025.21786710.3390/biom16010063