biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsTomoko Kawaimethylation profiling by arrayHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15728Acute promyelocytic leukemia (APL) is categorized as the M3 subtype in the French-American-British classification system. It represents a unique subtype of acute myeloid leukemia (AML) characterized by the t(15;17)/PML::RARA fusion, with leukemic cells arrested at the promyelocytic stage of myelopoiesis. Pediatric APL accounts for approximately 5%-10% of all AML. While the prognosis of APL patients has dramatically improved with the use of all-trans retinoic acid (ATRA) and arsenic trioxide, relapse patients still exist. To reveal the molecular biological basis of pediatric APL, we performed an integrative analysis of the trasncriptome and methylome.biostudies-arrayexpressHybridization - Hybridization to array was conducted according to the manufacturer's instructions.Scaning - Array scanning was performed with the iScan System according to the manufacture's instructions.Labeling - Labeling was conducted according to the manufacturer's instructions.Sample Collection - Acute promyelocytic leukemia (APL) is collected from Bon marrow or peripheral blood.Nucleic Acid Extraction - DNA was extracted from APL using the ALLPrep DNA/RNA Mini Kit (Qiagen, Hilden, Germany).MIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsTomoko KawaiData Transformation - Obtained idat files were processed using the ENmix package (v.1.38.1) in R software version 4.3.1. Beta values were obtained using “mpreprocess” in the ENmix package, and the manifest file was annotated using “IlluminaHumanMethylationEPICanno.ilm10b4.hg19.” 22,215 CpGs with percentage of low quality value greater than 0.05 were excluded. We also removed 19,681 probes located on the X or Y chromosome.falseMethylation profiling of pediatric acute promyelocytic leukemiaAcute promyelocytic leukemia (APL) is categorized as the M3 subtype in the French-American-British classification system. It represents a unique subtype of acute myeloid leukemia (AML) characterized by the t(15;17)/PML::RARA fusion, with leukemic cells arrested at the promyelocytic stage of myelopoiesis. Pediatric APL accounts for approximately 5%-10% of all AML. While the prognosis of APL patients has dramatically improved with the use of all-trans retinoic acid (ATRA) and arsenic trioxide, relapse patients still exist. To reveal the molecular biological basis of pediatric APL, we performed an integrative analysis of the trasncriptome and methylome.2026-08-01T00:00:00Z2026-08-01T01:01:06.621Z2025-10-15T11:46:51.759ZE-MTAB-15728EFO_0002944EFO_0003814EFO_0003813EFO_0002759EFO_0005518EFO_0003816EFO_0003815