{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Allison Roberts"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15740"],"description":["To identify genes involved in macrophage differentiation, a genome-wide CRISPR knockout library in macrophage progenitors was differentiated into macrophages. Samples were collected from the progenitor library at day 0, from a library maintained as progenitors for seven days, and from macrophages after seven days of differentiation."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Cells were lysed in 50mM Tris, 50mM EDTA, 1% SDS, pH 8 + 100ug/ml proteinase K, RNAse treated, then incubated with ammonium acetate for a final concentration of 1.875M. After centrifugation, supernatants were incubated with isopropanol to precipitate genomic DNA. After centrifugation gDNA pellets were washed in 70% ethanol. After a final centrifugation, gDNA pellets were air dried and resuspended in 10mM Tris HCL pH 8.5 + 0.1mM EDTA.","Sample Collection - The mouse Brie knockout CRISPR pooled library (Doench et al 2016) on the lentiGuide-Puro backbone was transduced into cas9-expressing conditionally-immortalized macrophage progenitors. Brie library transduced progenitors were differentiated into macrophages by removal of beta-estradiol and addition of MCSF. Cells were differentiated for seven days before harvest. Library samples were taken on day 0 of differentiation, and day 7 of differentiation. In addition, a sample of progenitors was not differentiated and passaged in parallel for 7 days. Three independent experiments were performed.","Library Construction - Library was constructed as previously described, using primers for the Brie library (Doench et al., 2016). Briefly the sgRNA loci were amplified by PCR using Illumina compatible primers that allow for sample multiplexing. Amplicons were purified then pooled.","Sequencing - Single-read (100bp) sequencing on an Illumina NovaSeq 6000"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - CRISPR sequence results were mapped and quantified using the R package MAGeCK (version 0.5.9.5). MAGeCK default mapping settings were used to automatically determine the trimming length and sgRNA length."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["DNA-seq"],"species":["Mus musculus"],"pubmed_title":["Genome-wide screen in Mycobacterium tuberculosis infected macrophages reveals innate regulation of antibacterial mediators by IRF2"],"pubmed_authors":["Allison Roberts","Jeffery Cox","Allison W. Roberts, Linda N. Del Cid, Nicholas E. Garelis, Jeffery S. Cox"],"additional_accession":[]},"is_claimable":false,"name":"Genome-wide CRISPR knockout screen to identify genes involved in macrophage differentiation","description":"To identify genes involved in macrophage differentiation, a genome-wide CRISPR knockout library in macrophage progenitors was differentiated into macrophages. Samples were collected from the progenitor library at day 0, from a library maintained as progenitors for seven days, and from macrophages after seven days of differentiation.","dates":{"release":"2026-04-29T00:00:00Z","modification":"2026-04-29T01:01:54.503Z","creation":"2025-10-15T14:47:49.032Z"},"accession":"E-MTAB-15740","cross_references":{"ENA":["ERP182243"],"EFO":["EFO_0002944","EFO_0004170","EFO_0002693","EFO_0005518","EFO_0003816","EFO_0004184"]}}