{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Remya Bhaskaran S"],"study_type":["transcription profiling by RT-PCR"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15757"],"description":["This study evaluated the expression of depression-associated genes (GAR1, PER3, MTPAP, SLC25A26, CD19) in whole-blood samples from 100 patients with depression and 100 healthy controls using SYBR Green-based quantitative RT-PCR on the Rotor-Gene Q system."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Scaning - qRT-PCR was performed using SYBR Green chemistry on the Rotor-Gene Q system (Qiagen). β-actin was used as an endogenous control.","Growth Protocol - Relative expression was calculated using the 2^-ΔCt method normalized to β-actin.","Nucleic Acid Extraction - Total RNA was isolated using TRIzol reagent (Thermo Fisher Scientific) following the manufacturer’s protocol.","Labeling - cDNA was synthesized from 1 µg of total RNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems).","Hybridization - qRT-PCR was performed using SYBR Green chemistry on the Rotor-Gene Q system (Qiagen). β-actin was used as an endogenous control.","Sample Treatment - Relative expression was calculated using the 2^-ΔCt method normalized to β-actin.","Sample Collection - Whole blood samples (5 mL) were collected in EDTA tubes from patients with depression and healthy controls following informed consent."],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Remya Bhaskaran S"],"data_protocol":["Data Transformation - Relative expression was calculated using the 2^-ΔCt method normalized to β-actin."],"additional_accession":[]},"is_claimable":false,"name":"Transcription profiling by RT-PCR of GAR1, PER3, MTPAP, SLC25A26, and CD19 in depression and control blood samples","description":"This study evaluated the expression of depression-associated genes (GAR1, PER3, MTPAP, SLC25A26, CD19) in whole-blood samples from 100 patients with depression and 100 healthy controls using SYBR Green-based quantitative RT-PCR on the Rotor-Gene Q system.","dates":{"release":"2025-11-03T00:00:00Z","modification":"2026-06-16T12:01:10.026Z","creation":"2025-10-16T12:27:49.588Z"},"accession":"E-MTAB-15757","cross_references":{"EFO":["EFO_0002944","EFO_0003814","EFO_0003813","EFO_0003789","EFO_0005518","EFO_0003816","EFO_0003815","EFO_0003969"]}}