biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsRemya Bhaskaran Stranscription profiling by RT-PCRHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15757This study evaluated the expression of depression-associated genes (GAR1, PER3, MTPAP, SLC25A26, CD19) in whole-blood samples from 100 patients with depression and 100 healthy controls using SYBR Green-based quantitative RT-PCR on the Rotor-Gene Q system.biostudies-arrayexpressScaning - qRT-PCR was performed using SYBR Green chemistry on the Rotor-Gene Q system (Qiagen). β-actin was used as an endogenous control.Growth Protocol - Relative expression was calculated using the 2^-ΔCt method normalized to β-actin.Nucleic Acid Extraction - Total RNA was isolated using TRIzol reagent (Thermo Fisher Scientific) following the manufacturer’s protocol.Labeling - cDNA was synthesized from 1 µg of total RNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems).Hybridization - qRT-PCR was performed using SYBR Green chemistry on the Rotor-Gene Q system (Qiagen). β-actin was used as an endogenous control.Sample Treatment - Relative expression was calculated using the 2^-ΔCt method normalized to β-actin.Sample Collection - Whole blood samples (5 mL) were collected in EDTA tubes from patients with depression and healthy controls following informed consent.MIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsRemya Bhaskaran SData Transformation - Relative expression was calculated using the 2^-ΔCt method normalized to β-actin.falseTranscription profiling by RT-PCR of GAR1, PER3, MTPAP, SLC25A26, and CD19 in depression and control blood samplesThis study evaluated the expression of depression-associated genes (GAR1, PER3, MTPAP, SLC25A26, CD19) in whole-blood samples from 100 patients with depression and 100 healthy controls using SYBR Green-based quantitative RT-PCR on the Rotor-Gene Q system.2025-11-03T00:00:00Z2026-06-16T12:01:10.026Z2025-10-16T12:27:49.588ZE-MTAB-15757EFO_0002944EFO_0003814EFO_0003813EFO_0003789EFO_0005518EFO_0003816EFO_0003815EFO_0003969