biostudies-arrayexpressYumeng PeiHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15766We wanted to explore the function of MG53 in THP-1 cells, so we treated THP-1 cells with control b-gal or MG53 adenovirus, and using PMA to induce THP-1 differentiated into macrophages. Thus exploring how MG53 affect macrophages.biostudies-arrayexpressSample Collection - THP-1 cells were grow in RPMI-1640, collected until the cell reached certain numbers.Nucleic Acid Extraction - RNA was extracted using RNA isolation kit (Vazyme, #RC112-01). The library construction was performed by Novogene company.Library Construction - Quality control was performed by Agilent 2100 bioanalyzer, and RNA was pooled according to effective concentration and then subjected to Illumina sequencing. Raw data of fasq format were processed through fastp software.Sequencing - FeatureCounts v1.5.0-p3 was used to count the reads numbers mapped to each gene. And FPKM was calculated based on the length of the gene and reads.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Firstly, reference genome sequences and gene model annotation files of relative species were downloaded from genome website, such as UCSC, NCBI, ENSEMBL. Secondly, Hisat2 (v2.2.1) was used to index reference genome sequence. Finally, clean data were aligned to reference genome via software Hisat2 (v2.2.1).MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XRNA-seq of total RNAHomo sapiensYumeng PeifalseRNA-seq of THP-1 cell lines overexpression of MG53We wanted to explore the function of MG53 in THP-1 cells, so we treated THP-1 cells with control b-gal or MG53 adenovirus, and using PMA to induce THP-1 differentiated into macrophages. Thus exploring how MG53 affect macrophages.2025-10-30T00:00:00Z2026-05-26T15:02:58.578Z2025-10-17T14:23:13.318ZE-MTAB-15766ERP182423EFO_0002944EFO_0004170EFO_0009653EFO_0005518EFO_0003816EFO_0004184