{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Tomoko Kawai"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15773"],"description":["Acute promyelocytic leukemia (APL) is categorized as the M3 subtype in the French-American-British classification system. It represents a unique subtype of acute myeloid leukemia (AML) characterized by the t(15;17)/PML::RARA fusion, with leukemic cells arrested at the promyelocytic stage of myelopoiesis. Pediatric APL accounts for approximately 5%-10% of all AML. While the prognosis of APL patients has dramatically improved with the use of all-trans retinoic acid (ATRA) and arsenic trioxide, relapse patients still exist. To reveal the molecular biological basis of pediatric APL, we performed an integrative analysis of the trasncriptome and methylome."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - Sequencing libraries were prepared using an SureSelect XT HS2 mRNA Library Preparation System (Agilent Technologies, Santa Clara, CA).","Nucleic Acid Extraction - Total RNA was extracted using the ALLPrep DNA/RNA Mini Kit (Qiagen, Hilden, Cermany).","Sequencing - 150 bp paired end sequencing with Illumina Hiseq X Ten","Sample Collection - Acute promyelocytic leukemia (APL) is collected from Bon marrow or peripheral blood."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - We obtained gene counts from alignment-based transcriptome quantification based on gencode v40 using StringTie (v.2.1.5).","Sequence Alignment - Filtering low-quality reads, adaptor trimming, and deduplication of the FASTQ reads were performed using fastp (v.1.0.1). Preprocessed reads were aligned to the genomic hg38 using STAR (v.2.7.10a)."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["HiSeq X Ten"],"study_type":["RNA-seq of coding RNA"],"species":["Homo sapiens"],"pubmed_authors":["Tomoko Kawai"],"additional_accession":[]},"is_claimable":false,"name":"Gene expression profiling of pediatric acute promyelocytic leukemia","description":"Acute promyelocytic leukemia (APL) is categorized as the M3 subtype in the French-American-British classification system. It represents a unique subtype of acute myeloid leukemia (AML) characterized by the t(15;17)/PML::RARA fusion, with leukemic cells arrested at the promyelocytic stage of myelopoiesis. Pediatric APL accounts for approximately 5%-10% of all AML. While the prognosis of APL patients has dramatically improved with the use of all-trans retinoic acid (ATRA) and arsenic trioxide, relapse patients still exist. To reveal the molecular biological basis of pediatric APL, we performed an integrative analysis of the trasncriptome and methylome.","dates":{"release":"2026-08-01T00:00:00Z","modification":"2026-08-01T01:01:06.524Z","creation":"2025-10-17T21:22:53.913Z"},"accession":"E-MTAB-15773","cross_references":{"EFO":["EFO_0002944","EFO_0004170","EFO_0004917","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184"]}}