biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsWei HongIllumina NovaSeq XRNA-seq of coding RNAClostridium difficile 630Clostridium difficile 630https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15780To reveal the effects of deleting the ΔsigV and Δanti-sigV on clindamycin tolerance in Clostridioides difficile, we first constructed knockout mutants of the sigV and anti-sigV genes and then examined the resulting phenotypic changes, with a particular focus on alterations in clindamycin tolerance. To further elucidate the mechanism underlying the observed changes in clindamycin tolerance, we performed transcriptomic data analysis to identify how sigV and anti-sigV regulate clindamycin-related pathways.biostudies-arrayexpressNucleic Acid Extraction - Total RNA was extracted from WT, ΔsigV, and Δanti-sigV using a bacterial total RNA extraction kit (DP430, TIANGEN, Beijing).Library Construction - Once the samples were deemed satisfactory, the following steps were conducted for sequencing: (1) Removal of ribosomal RNA; (2) Enrichment and purification of mRNA; (3) Fragmentation of mRNA; (4) Construction of the sequencing library and quality assessment of the library.Sequencing - The sequencing library was then sequenced on the lllumina NovaSeqXPlus platform.Sample Collection - The WT, ΔsigV, and Δanti-sigV strains were inoculated into BHIS medium and cultured until an OD600 of 0.6 was reached. The cultures were then centrifuged at 13,500 × g for 10 min, the supernatant was discarded, and the bacterial pellets of each strain were collected. The samples were transported with sufficient dry ice in a foam box to Shanghai Majorbio Bio-Pharm Technology Co., Ltd. for transcriptome sequencing.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesWei HongfalseRNA-seq of Clostridioides difficile ΔsigV、Δanti-sigV against wild-type strain controlTo reveal the effects of deleting the ΔsigV and Δanti-sigV on clindamycin tolerance in Clostridioides difficile, we first constructed knockout mutants of the sigV and anti-sigV genes and then examined the resulting phenotypic changes, with a particular focus on alterations in clindamycin tolerance. To further elucidate the mechanism underlying the observed changes in clindamycin tolerance, we performed transcriptomic data analysis to identify how sigV and anti-sigV regulate clindamycin-related pathways.2025-12-31T00:00:00Z2025-12-31T02:02:53.847Z2025-10-20T10:44:56.601ZE-MTAB-15780ERP182492EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184