biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsZhiwei WangTRIzol reagent kit, NanoDrop 2000 spectrophotometerNEBNext Ultra II Directional RNA Library Prep Kit, Agilent Bioanalyzer 2100Not applicableIllumina NovaSeq 6000Field cultivation site in Beijing, ChinaRNA-seq of coding RNAToona sinensisToona sinensishttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15781Toona sinensis, a medicinal and edible plant, holds economic and ecological significance in Asia. Despite its diverse applications, the genetic basis of leaf color formation remains unclear. This study aimed to investigate the molecular mechanisms underlying color variations across three cultivars (red, brown, green) at different developmental stages. Young tender leaflets from apical tips and fully expanded leaflets from basal positions of the same compound leaf were collected for RNA-Seq analysis.biostudies-arrayexpressGrowth Protocol - Toona sinensis plants were grown under natural field conditions in a cultivation site in Beijing, China, with standard agricultural practices. Samples were collected during the spring growing season when leaf flushing occurred.Sequencing - Nucleic acid sequencing protocol: Sequencing was performed on an Illumina NovaSeq 6000 platform with paired-end 150 bp (PE150) reads in high-throughput mode.Sample Collection - Young tender leaflets from apical tips and fully expanded leaflets from basal positions of the same compound leaf were collected from three Toona sinensis cultivars exhibiting distinct leaf colors: red, brown, and green. Tissues were immediately frozen in liquid nitrogen and stored at -80°C until RNA extraction.Nucleic Acid Extraction - Total RNA was extracted using the TRIzol reagent (Invitrogen) according to the manufacturer’s instructions. RNA concentration and purity were determined using a NanoDrop spectrophotometer (OD260/280 ratio), and integrity was assessed by agarose gel electrophoresis.Library Construction - Stranded RNA-seq libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) following the standard protocol. Poly(A) selection was performed to enrich for mRNA. Libraries were amplified with 12 PCR cycles and size-selected for fragments around 300 bp. Library quality and concentration were validated using an Agilent Bioanalyzer.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesZhiwei WangfalseTranscriptomic Analysis of Leaf Color Development in Toona sinensis: Insights into the Molecular Mechanisms of Pigmentation in Three CultivarsToona sinensis, a medicinal and edible plant, holds economic and ecological significance in Asia. Despite its diverse applications, the genetic basis of leaf color formation remains unclear. This study aimed to investigate the molecular mechanisms underlying color variations across three cultivars (red, brown, green) at different developmental stages. Young tender leaflets from apical tips and fully expanded leaflets from basal positions of the same compound leaf were collected for RNA-Seq analysis.2025-10-31T00:00:00Z2025-10-31T02:02:03.108Z2025-10-20T10:52:51.232ZE-MTAB-15781ERP182493EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003738EFO_0004184