{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Yumeng Pei"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15805"],"description":["We performed Cleavage Under Targets and Release Using Nuclease (CUT&RUN) assays in BMDMs, deciphered MG53's binding site."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - According to the manual, this step including DNA damage repair and end preparation, adapter ligation and library amplification.","Sample Collection - According to the kit from Vazyme (),BMDMs were digest with trypsin, collect the cell suspension, centrifuge, and discard the medium to collect cells.","Nucleic Acid Extraction - Binding of cells to ConA Beads Pro, and the judgment can be performed according to the actual condition.","Sequencing - The library size distribution analysis can be performed by devices based on electrophoretic separation such as LabChip GX, GXII, GX Touch (PerkinElmer); Bioanalyzer, Tapestation (Agilent Technologies); and Fragment Analyzer (Advanced Analytical). A CUT&RUN assay is performed for histone H3K4me3 using 100,000 K562 cells."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Sequencing data analysis, transcription start site (TSS) enrichment, and Integrative Genomics Viewer (IGV) views: CUT&RUN assays are performed for histone H3K4me3 and transcription factor CTCF using 100,000 K562 cells."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina HiSeq X"],"study_type":["CUT&RUN"],"species":["Mus musculus"],"pubmed_authors":["Yumeng Pei"],"additional_accession":[]},"is_claimable":false,"name":"CUT&RUN for MG53 in BMDMs","description":"We performed Cleavage Under Targets and Release Using Nuclease (CUT&RUN) assays in BMDMs, deciphered MG53's binding site.","dates":{"release":"2025-11-16T00:00:00Z","modification":"2026-05-27T12:36:51.62Z","creation":"2025-10-21T15:31:18.308Z"},"accession":"E-MTAB-15805","cross_references":{"ENA":["ERP182589"],"EFO":["EFO_0002944","EFO_0009973","EFO_0004170","EFO_0005518","EFO_0003816","EFO_0004184"]}}