biostudies-arrayexpressYumeng PeiMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15805We performed Cleavage Under Targets and Release Using Nuclease (CUT&RUN) assays in BMDMs, deciphered MG53's binding site.biostudies-arrayexpressLibrary Construction - According to the manual, this step including DNA damage repair and end preparation, adapter ligation and library amplification.Sample Collection - According to the kit from Vazyme (),BMDMs were digest with trypsin, collect the cell suspension, centrifuge, and discard the medium to collect cells.Nucleic Acid Extraction - Binding of cells to ConA Beads Pro, and the judgment can be performed according to the actual condition.Sequencing - The library size distribution analysis can be performed by devices based on electrophoretic separation such as LabChip GX, GXII, GX Touch (PerkinElmer); Bioanalyzer, Tapestation (Agilent Technologies); and Fragment Analyzer (Advanced Analytical). A CUT&RUN assay is performed for histone H3K4me3 using 100,000 K562 cells.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Sequencing data analysis, transcription start site (TSS) enrichment, and Integrative Genomics Viewer (IGV) views: CUT&RUN assays are performed for histone H3K4me3 and transcription factor CTCF using 100,000 K562 cells.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina HiSeq XCUT&RUNMus musculusYumeng PeifalseCUT&RUN for MG53 in BMDMsWe performed Cleavage Under Targets and Release Using Nuclease (CUT&RUN) assays in BMDMs, deciphered MG53's binding site.2025-11-16T00:00:00Z2026-05-27T12:36:51.62Z2025-10-21T15:31:18.308ZE-MTAB-15805ERP182589EFO_0002944EFO_0009973EFO_0004170EFO_0005518EFO_0003816EFO_0004184