biostudies-arrayexpressMohamed Abdel-MohsenHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15808RNAseq profilling of monocytes co-cultured with HIV-TYBE infected primary CD4 T cells (E:T=10:1) in the presence of 10-1074, 10-1074-SiaD or Isotype antibodybiostudies-arrayexpressLibrary Construction - Libraries were constructed on Stranded mRNA Library Prep Kit (Illumina) using 25 ng input RNASequencing - The sequencing was performed on a NovaSeq X Plus 10B flowcell as 50bp single-end, and a minimum of 30M reads were captured for each sampleSample Collection - Monocytes co-cultured with autologous HIV-infected CD4+ in the presence of 50 nM isotype control, 10-1074 and 10-1074-SiaD for 16 h were harvested.Nucleic Acid Extraction - RNA isolation was performed using the using RNAeasy micro kit (Qiagen, Catalog #74004) with on-column DNA digestionOrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - The RNA-seq data analysis was performed by Bencos Research Solutions Pvt. Ltd., Mumbai, India. For the analysis, the raw data obtained after performing the RNA sequencing were aligned to Reference Human Genome assembly GRCh38 using the salmon (v 1.10.1) software in conjunction with STAR aligner (v 2.7.10a). Raw read counts were used as the input to perform differential gene expression analysis using the R package DESeq2 (v 1.34.0). DESeq2 normalized read counts were used to calculate the log2 fold change and adjusted p-values. P values were adjusted using Benjamini-Hochberg procedure to determine the False Discovery Rate (FDR) and control for multiple testingData Transformation - The RNA-seq data analysis was performed by Bencos Research Solutions Pvt. Ltd., Mumbai, India. For the analysis, the raw data obtained after performing the RNA sequencing were aligned to Reference Human Genome assembly GRCh38 using the salmon (v 1.10.1) software in conjunction with STAR aligner (v 2.7.10a).UnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XRNA-seq of coding RNAHomo sapiensHIV-Induced Sialoglycans on Infected CD4+ T Cells Promote Immune Evasion from Myeloid Cell-Mediated KillingMohamed Abdel-MohsenShalini Singh, S. M. Shamsul Islam, Rui Liu, Opeyemi S. Adeniji, Lacy M. Simons, Pratima Saini, Hiroaki Tateno, Ali Danesh, Paul W. Denton, Leila B. Giron, R. Brad Jones, Judd F. Hultquist, Han Xiao, Mohamed Abdel-MohsenfalseHIV-Induced Sialoglycans on Infected CD4+ T Cells Promote Immune Evasion from Myeloid Cell-Mediated KillingRNAseq profilling of monocytes co-cultured with HIV-TYBE infected primary CD4 T cells (E:T=10:1) in the presence of 10-1074, 10-1074-SiaD or Isotype antibody2025-10-15T00:00:00Z2025-10-15T13:33:40.579Z2025-10-15T11:16:45.173ZE-MTAB-15808ERP182215EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184