biostudies-arrayexpressTongtong WangMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15819Brown adipocyte-specific ChREBP knockout were generated by crossing mice with a floxed exon1a in the Mlxipl gene with Ucp1-Cre mice. These ChREBPflox/flox Ucp1-Cre+ (Cre+) mice and ChREBP-expressing control (Cre-) mice were studied under various conditions of BAT activation. Housing at room temperature (22°C, RT) was chosen as a control condition when BAT is mildly activated. Cold-exposure (6°C) was carried out for 1 day (acute cold ), as an early stage when proliferative processes of BAT adaptation are not yet taking place, or for 10 days (chronic cold) when BAT is fully expanded.biostudies-arrayexpressSequencing - the sequencing was performed at NovaSeq 6000 platform S4 flow cell (Illumina, San Diego) with PE150 strategy.Nucleic Acid Extraction - Nuclei were loaded on a 10x Chip G directlyLibrary Construction - 10x 3' V3.1 library constructionGrowth Protocol - noneSample Treatment - cold exposureSample Collection - Frozen adipose tissue was minced into 1–3 mm pieces on ice. The minced tissue was homogenized in a Dounce homogenizer on ice in 0.1% CHAPS in CST buffer with 0.2U Rnase inhibitor, lysed for 5 min, and quenched by 1% BSA in PBS with 0.2U Rnase inhibitor. The homogenized adipose tissue was filtered through a 40 μm cell strainer and centrifuged at 500 x g for 5 min at 4°C. The pellet was resuspended and washed with 1% BSA in PBS with 0.2U Rnase inhibitor. The nuclei suspension was centrifuged again at 500 x g for 5 min at 4°C, resuspended in 1% BSA containing PBS with 1U Rnase inhibitor, and filtered through a 20 μm strainers.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Cell Ranger (v7.1.0) was used for demultiplexing and alignment to the reference genome GRCm38 (mm10). Single-nucleus mRNA-sequencing (snRNA-seq) data were processed using the Seurat [89] package (v5.1) in R. For quality control, nuclei with more than 15% mitochondrial reads, more than 25,000 mRNA counts or more than 5,000 detected genes were excluded. Genes found in fewer than 3 cells were removed. After quality control filtering, an average of 5,778 nuclei per sample were retained, with a median per-nucleus complexity across all samples of 2,163 features and 4,811 UMIs. Six samples were integrated using the Harmony (v1.2.3) algorithm, with default parameters and sample ID as a grouping variable.Sequence Alignment - FASTQ files extracted from tar archives Alignment and quantification with Cell Ranger count (10x Genomics), parameters: --nosecondary --no-bamMetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000single nucleus RNA sequencingMus musculusSingle-nucleus mRNA-sequencing reveals dynamics of lipogenic and thermogenic adipocyte populations in murine brown adipose tissue in response to cold exposureJanina Behrens, Tongtong Wang, Christoph Kilian, Anna Worthmann, Joerg Heeren, Lorenz Adlung, Ludger SchejaTongtong WangfalseSingle-nucleus mRNA-sequencing reveals dynamics of lipogenic and thermogenic adipocyte populations in murine brown adipose tissue in response to cold exposureBrown adipocyte-specific ChREBP knockout were generated by crossing mice with a floxed exon1a in the Mlxipl gene with Ucp1-Cre mice. These ChREBPflox/flox Ucp1-Cre+ (Cre+) mice and ChREBP-expressing control (Cre-) mice were studied under various conditions of BAT activation. Housing at room temperature (22°C, RT) was chosen as a control condition when BAT is mildly activated. Cold-exposure (6°C) was carried out for 1 day (acute cold ), as an early stage when proliferative processes of BAT adaptation are not yet taking place, or for 10 days (chronic cold) when BAT is fully expanded.2025-11-10T00:00:00Z2026-05-27T14:44:24.045Z2025-10-22T14:25:32.999ZE-MTAB-15819ERP182709EFO_0002944EFO_0004170EFO_0003789EFO_0004917EFO_0009809EFO_0005518EFO_0003816EFO_0004184EFO_000396910.1016/j.molmet.2025.102252