biostudies-arrayexpressYumeng PeiMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15824Using MG53-TG and MG53-KO mice derived BMDMs to operate RNAseq.biostudies-arrayexpressNucleic Acid Extraction - RNA was extracted using RNA isolation kit (Vazyme, #RC112-01). The library construction was performed by Novogene company.Library Construction - Quality control was performed by Agilent 2100 bioanalyzer, and RNA was pooled according to effective concentration and then subjected to Illumina sequencing. Raw data of fasq format were processed through fastp software.Sequencing - FeatureCounts v1.5.0-p3 was used to count the reads numbers mapped to each gene. And FPKM was calculated based on the length of the gene and reads.Sample Collection - BMDMs were derived from different genotype mouse and then collect using kit (Vazyme #R323).OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Firstly, reference genome sequences and gene model annotation files of relative species were downloaded from genome website, such as UCSC, NCBI, ENSEMBL. Secondly, Hisat2 (v2.2.1) was used to index reference genome sequence. Finally, clean data were aligned to reference genome via software Hisat2 (v2.2.1).MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XRNA-seq of coding RNAMus musculusYumeng PeifalseMG53 TG and MG53 KO mice BMDMs compared to their littermates WTUsing MG53-TG and MG53-KO mice derived BMDMs to operate RNAseq.2025-11-16T00:00:00Z2026-05-27T16:46:25.717Z2025-10-22T16:20:14.55ZE-MTAB-15824ERP182727EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184