biostudies-arrayexpressFrancesca DemichelisHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15836Gene fusions between the ETS family transcription factor ERG and the regulatory region of an androgen receptor (AR)-regulated gene (mainly TMPRSS2) characterize about 50% of prostate cancers (PCa) and represent early somatic events that co-initiate tumorigenesis. Yet, the molecular programs unleashed by ERG at disease onset remain elusive. Here, we used an inducible, temporally controlled system to express ERG in prostate cells (LNCaP and 22Rv1) for the accurate mapping of early ERG-driven processes.biostudies-arrayexpressGrowth Protocol - 22Rv1 (CVCL_1045) and LNCaP-FGC (CVCL_1379) were grown in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS) (Gibco), 1% L- glutamine (Gibco), and 1% penicillin-streptomycin (Gibco).Sample Collection - Total cellular RNA was extracted using the RNeasy mini kit (Qiagen).Library Construction - Poly-A-containing mRNA isolation and library preparation were performed by using the TruSeq Stranded mRNA Library Prep (Illumina) and the AMPure XP SPRI Reagent (Beckman Coulter).Sequencing - Libraries were sequenced with the HiSeq sequencing system (Illumina) using the HiSeq® Rapid SR Kit v2 (100 cycles, Illumina).Nucleic Acid Extraction - Total cellular RNA was extracted using the RNeasy mini kit (Qiagen) including on-column DNase I digestion and quantified using the Qubit RNA Broad-Range Assay Kit (Thermo Fisher Scientific).Sample Treatment - For DOX-inducible overexpression, the gene of interest was cloned into pCW57.1 (#41393, Addgene). Cells were induced with full medium containing EtOH or DOX for 4 days (medium changed at 2 days) or 1 day.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - Reads were trimmed based on quality using trimmomatic and aligned against the human reference genome hg38 using STAR (version 2.7.2b).Data Transformation - Raw counts are provided in the genecount_matrix.txt file. Raw counts are obtained using the --quantMode option of STAR aligner.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina HiSeq 2500RNA-seq of coding RNAHomo sapiensFrancesca DemichelisfalseRNA-seq of Human Prostate Cancer cell lines (LNCaP and 22RW1) with T2ERG expressionGene fusions between the ETS family transcription factor ERG and the regulatory region of an androgen receptor (AR)-regulated gene (mainly TMPRSS2) characterize about 50% of prostate cancers (PCa) and represent early somatic events that co-initiate tumorigenesis. Yet, the molecular programs unleashed by ERG at disease onset remain elusive. Here, we used an inducible, temporally controlled system to express ERG in prostate cells (LNCaP and 22Rv1) for the accurate mapping of early ERG-driven processes.2026-06-10T00:00:00Z2026-06-10T01:00:55.573Z2025-10-23T16:22:57.643ZE-MTAB-15836ERP182836EFO_0002944EFO_0004170EFO_0003789EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184EFO_0003969