biostudies-arrayexpressElisabeth De LeeuwMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15850scRNAseq experiment of mouse dendritic cells in the lung after intranasal infection with pneumonia virus of mice (7 days postnatally) or mock and/or intranasal house dust mite or PBS sensitisation (14 days postnatally). Cells were isolated when mice were 15 days old. No prior bronchoalveolar lavage was taken and mice received intravenous labeling of immune cells with CD45 to distuinguish between circulating and lung resident immune cells.biostudies-arrayexpressSequencing - Sequencing libraries were loaded on an Illumina NovaSeq flow cell at VIB Nucleomics core with sequencing settings according to the recommendations of 10x GenomicsSample Collection - Single cell suspensions of the lung were obtained and cells were stained for 30 minutes at 4 degrees celcius before sorting. Following sorting strategy was used: live cells, CD45 IV negative cells. For each sample, 7500 immune cells were sorted (CD45+) and 7500 structural cells (CD45-, CD31-, Ter119-, EPCAM+)Library Construction - The scRNA-Seq libraries were prepared using the GemCode Single Cell 3’ Gel Bead and Library kit, version NextGEM 3.1 (10x Genomics) according to the manufacturer’s instructionsNucleic Acid Extraction - Single live cells were sorted and loaded onto a Chromium GemCode Single Cell Instrument (10x Genomics) to generate single-cell gel beads in emulsion (GEMs)OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Data normalisation and transformation was performed according to standard Seurat vignettes. The DC were subsetted after differential gene expression analysisUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNA from single cellsMus musculusSam DupontElisabeth De LeeuwBart LambrechtfalseEffect of the combined neonatal pneumonia virus of mice infection and house dust mite exposure on the trancriptional profile of dendritic cells in the lungscRNAseq experiment of mouse dendritic cells in the lung after intranasal infection with pneumonia virus of mice (7 days postnatally) or mock and/or intranasal house dust mite or PBS sensitisation (14 days postnatally). Cells were isolated when mice were 15 days old. No prior bronchoalveolar lavage was taken and mice received intravenous labeling of immune cells with CD45 to distuinguish between circulating and lung resident immune cells.2025-10-22T00:00:00Z2026-05-27T17:38:21.084Z2025-10-24T13:21:30.325ZE-MTAB-15850ERP182888EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0003816EFO_0004184