biostudies-arrayexpressSandhya Visweswariah SMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15862Organoids were prepared from the small intestine of wild type C57BL6N mice and mice harbouring an activating mutation in Gucy2c that converts a serine at position 839 to an isoleucine. Organoids were treated with 1 micromolar uroguanylin for 18h. RNA was prepared and subjected to RNAseq analysis.biostudies-arrayexpressSequencing - Illumina NovaSeq used for sequencing with a minimum of 20 million reads with polyA selection. Sequencing performed by Azenta. Illumina Nova Seq used.Sample Collection - Organoids were cultured for 3 days prior to treatment with uroguanylin (1 micro molar) or medium alone. 18h following treatment, organoids were harvested in DMEM:f12 medium and RNA prepared.Growth Protocol - Organoids were cultured for 3 days prior to RNA extractionLibrary Construction - mRNA fragment and synthesis of cDNA followed by end repair and dA tailing. cDNA purified and amplified by PCRNucleic Acid Extraction - Total RNA purified form cultured organoids using Invitrogen RNA purification kits. RNA was treated with DNAse on the column.Sample Treatment - Organoids prepared from wild type and S839I mice were cultured for 3 days after passaging and then treated with uroguanylin for 16h.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - Sequence reads were trimmed to remove possible adapter sequences and nucleotides with poor quality using Trimmomatic v.0.36. The trimmed reads were mapped to the Mus musculus GRCm38 reference genome available on ENSEMBL using the STAR aligner v.2.5.2b. The STAR aligner is a splice aligner that detects splice junctions and incorporates them to help align the entire read sequences. BAM files were generated as a result of this step. Below are the statistics of mapping the reads to the reference genome.Data Transformation - Reads were trimmed, mapped on to the mouse genome and differential gene expression calculated by deepseq2.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XRNA-seq of coding RNAMus musculusSandhya Visweswariah SfalseRNAseq of small intestinal organoids prepared from wild type mice and mice harbouring a mutation in Gucy2c at position Ser839 that converts it to an isoleucine following treatment with uroguanylinOrganoids were prepared from the small intestine of wild type C57BL6N mice and mice harbouring an activating mutation in Gucy2c that converts a serine at position 839 to an isoleucine. Organoids were treated with 1 micromolar uroguanylin for 18h. RNA was prepared and subjected to RNAseq analysis.2026-06-30T00:00:00Z2026-06-30T01:01:12.396Z2025-10-24T15:41:10.526ZE-MTAB-15862ERP182993EFO_0002944EFO_0004170EFO_0003789EFO_0004917EFO_0005518EFO_0003816EFO_0004184EFO_0003969