biostudies-arrayexpressGrace TanOryza sativahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15888lo determine transcriptional response to GS-inhibiting herbicides, RNAseq analysis was perform on rice leaf discs treated with 10mM glutamine, 100uM phosphinothricin, or 5uM methionine sulfoxime for 48h. Treatment was performed by floating leaf discs in MS basal media with no sucrose in the light at 28C.biostudies-arrayexpressSample Treatment - Rice leaf discs were mature leaf sections were floated abaxial side down in liquid ½ MS solution (pH 5.7, no sucrose) containing the chemical of interest and incubated for 48h at 28°C, under 24h light.Library Construction - Directional mRNA library preparation (poly A enrichment)Sample Collection - Leaf samples were blotted dry and snap-frozen in liquid nitrogenSequencing - RNA extracts were submitted to Novogene, where sequencing was done on an Illumina NovaSeq 6000Growth Protocol - Rice plants were grown in glasshouse under natural light until maturity.Nucleic Acid Extraction - Frozen samples are milled in extraction buffer and total RNA extracted using the QIAGEN Plant RNeasy kitOrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - Reads were aligned to O. sativa Phytozome v7 reference genome.Data Transformation - Read counts data was normalised via log transformationMetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAOryza sativaCationic amino acid transporters (CAT) mediate accumulation and sensitivity to the systemic herbicide L-phosphinothricinGrace TanGrace Zi Hao Tan,, Herrick Yu Kan Koh, Zheng Yong Poh, Yuri Trusov, Shalini Krishnamoorthi, Kasey Goh, Célio Cabral Oliveira, José Ramón Botella, Daisuke UranoDaisuke UranofalseResponse of rice treated with glutamine and GS-inhibitorslo determine transcriptional response to GS-inhibiting herbicides, RNAseq analysis was perform on rice leaf discs treated with 10mM glutamine, 100uM phosphinothricin, or 5uM methionine sulfoxime for 48h. Treatment was performed by floating leaf discs in MS basal media with no sucrose in the light at 28C.2025-11-05T00:00:00Z2026-05-28T11:43:15.181Z2025-11-05T14:32:27.742ZE-MTAB-15888ERP183668EFO_0002944EFO_0004170EFO_0003789EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184EFO_0003969