biostudies-arrayexpressGrace TanKlebsormidium nitenshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15889To determine transcriptional response to GS-inhibiting herbicides, RNAseq analysis was perform on Klebsormidium treated with 10mM glutamine, 100uM phosphinothricin, or 5uM methionine sulfoxime for 48h. Treatment was performed by incubating cultures with the compounds in Full Yamagami media under low light at 22C with shaking at 98rpm.biostudies-arrayexpressSample Collection - Samples were harvested via centrifugation, washed once in sterile MilliQ water, and snap-frozen in liquid nitrogenLibrary Construction - Directional mRNA library preparation (poly A enrichment)Growth Protocol - Cell suspensions were precultured in liquid Full Yamagami media for seven days prior to treatmentSequencing - RNA extracts were submitted to Novogene, where sequencing was done on an Illumina NovaSeq 6000Nucleic Acid Extraction - Frozen samples are milled in extraction buffer and total RNA extracted using the QIAGEN Plant RNeasy kitSample Treatment - Klebsormidium cultures were inoculated to liquid Full Yamagami media (pH5.5, 1% sucrose) containing the chemical of interest and grown for 48h at 80rpm, at 22°C, under 24h of low light.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - Reads were aligned to K. nitens v1.1 reference genome.Data Transformation - Read counts data was normalised via log transformationMetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAKlebsormidium nitensCationic amino acid transporters (CAT) mediate accumulation and sensitivity to the systemic herbicide L-phosphinothricinGrace TanGrace Zi Hao Tan,, Herrick Yu Kan Koh, Zheng Yong Poh, Yuri Trusov, Shalini Krishnamoorthi, Kasey Goh, Célio Cabral Oliveira, José Ramón Botella, Daisuke UranoDaisuke UranofalseResponse of Klebsormidium treated with glutamine and GS-inhibitorsTo determine transcriptional response to GS-inhibiting herbicides, RNAseq analysis was perform on Klebsormidium treated with 10mM glutamine, 100uM phosphinothricin, or 5uM methionine sulfoxime for 48h. Treatment was performed by incubating cultures with the compounds in Full Yamagami media under low light at 22C with shaking at 98rpm.2025-10-31T00:00:00Z2025-10-31T02:02:01.655Z2025-10-28T09:24:03.673ZE-MTAB-15889ERP183179EFO_0002944EFO_0004170EFO_0003789EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184EFO_0003969