biostudies-arrayexpressGrace TanArabidopsis thalianahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15892Transcriptomes of Arabidopsis knockout mutants for cationic amino acid transporters (CATs) were assessed under normal growth conditions to determine the disruption to normal metabolism caused by the mutations.biostudies-arrayexpressSample Collection - Samples were harvested, washed once in sterile MilliQ water, and snap-frozen in liquid nitrogenLibrary Construction - Frozen samples are milled in extraction buffer and total RNA extracted using the QIAGEN Plant RNeasy kitSequencing - RNA extracts were submitted to Novogene, where sequencing was done on an Illumina NovaSeq 6000Growth Protocol - Seeds were surface-sterilised and stratified for three days in the dark at 4C, then germinated on full-strength MS media (1% sucrose, 1% agar, pH5.8) for eight days at 22C under 24h lightSample Treatment - Seedlings were transferred from solid media to liquid half-strength MS Basal (pH5.7) media and incubated at 22°C for 48h underNucleic Acid Extraction - Frozen samples are milled in extraction buffer and total RNA extracted using the QIAGEN Plant RNeasy kitOrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - Reads were aligned to Arabidopsis TAIR10 reference genome.Data Transformation - Read counts data was normalised via log transformationMetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAArabidopsis thalianaCationic amino acid transporters (CAT) mediate accumulation and sensitivity to the systemic herbicide L-phosphinothricinGrace TanGrace Zi Hao Tan,, Herrick Yu Kan Koh, Zheng Yong Poh, Yuri Trusov, Shalini Krishnamoorthi, Kasey Goh, Célio Cabral Oliveira, José Ramón Botella, Daisuke UranoDaisuke UranofalseBasal transcriptome of Arabidopsis amino acid transporter mutantsTranscriptomes of Arabidopsis knockout mutants for cationic amino acid transporters (CATs) were assessed under normal growth conditions to determine the disruption to normal metabolism caused by the mutations.2025-10-30T00:00:00Z2026-05-30T16:54:58.705Z2025-10-28T12:24:58.372ZE-MTAB-15892ERP183194EFO_0002944EFO_0004170EFO_0003789EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184EFO_0003969